單細胞測序揭示下丘腦細胞對社會行為的控制

發布時間：2019-10-21 11:41 原文鏈接：單細胞測序揭示下丘腦細胞對社會行為的控制

　　美國加州理工學院David J. Anderson小組取得一項新進展，他們利用單細胞測序對控制社會行為下丘腦節點的細胞類型進行了多模式分析。相關論文發表在2019年10月17日出版的《細胞》雜志上。

　　研究人員介紹，腹內側下丘腦的腹側分支（VMHvl）包含約4000個神經元，這些神經元投射到多個目標并控制與生俱來的社會行為，包括侵略和坐騎。然而，VMHv1中的細胞類型的數目及其與連通性和行為功能的關系是未知的。

　　研究人員使用了兩個獨立的平臺：SMART-seq（約4500個神經元）和10x（約78000個神經元）進行了單細胞RNA測序，并分別研究了轉錄組學特征與軸突預測或行為激活之間的對應關系。典型相關分析（CCA）確定了17個轉錄組類型（T類型），包括幾個性雙態群體，其中大多數已通過seqFISH驗證。

　　早期基因分析確定了對入侵者雄性和雌性表現出優先反應的T類型，但僅是行為特異性激活的罕見例子。出乎意料的是，許多VMHvl T類型包含具有不同投影目標偏好的混合神經元群體。總體而言，這些分析表明，令人驚訝的是，很少有VMHvl T類型表現出與特定于行為的激活和連通性明確的對應關系。

　　附：英文原文

　　Title: Multimodal Analysis of Cell Types in a Hypothalamic Node Controlling Social Behavior

　　Author: Dong-Wook Kim, Zizhen Yao, Lucas T. Graybuck, Tae Kyung Kim, Thuc Nghi Nguyen, Kimberly A. Smith, Olivia Fong, Lynn Yi, Noushin Koulena, Nico Pierson, Sheel Shah, Liching Lo, Allan-Hermann Pool, Yuki Oka, Lior Pachter, Long Cai, Bosiljka Tasic, Hongkui Zeng, David J. Anderson

　　Issue&Volume: 2019/10/17

　　Abstract:

　　The ventrolateral subdivision of the ventromedial hypothalamus (VMHvl) contains ～4,000 neurons that project to multiple targets and control innate social behaviors including aggression and mounting. However, the number of cell types in VMHvl and their relationship to connectivity and behavioral function are unknown. We performed single-cell RNA sequencing using two independent platforms—SMART-seq (～4,500 neurons) and 10x (～78,000 neurons)—and investigated correspondence between transcriptomic identity and axonal projections or behavioral activation, respectively. Canonical correlation analysis (CCA) identified 17 transcriptomic types (T-types), including several sexually dimorphic clusters, the majority of which were validated by seqFISH. Immediate early gene analysis identified T-types exhibiting preferential responses to intruder males versus females but only rare examples of behavior-specific activation. Unexpectedly, many VMHvl T-types comprise a mixed population of neurons with different projection target preferences. Overall our analysis revealed that, surprisingly, few VMHvl T-types exhibit a clear correspondence with behavior-specific activation and connectivity.

　　DOI: 10.1016/j.cell.2019.09.020

　　Source: https://www.cell.com/cell/fulltext/S0092-8674(19)31071-2

　　期刊信息

　　Cell：《細胞》，創刊于1974年。隸屬于細胞出版社，最新IF：36.216

　　官方網址：https://www.cell.com/

　　投稿鏈接：https://www.editorialmanager.com/cell/default.aspx