摘要
背景：對測定血清肌酐濃度的Jaffe法進行定標時，應用一種以血清為基礎并添加可以償Jaffe法標準品中干擾的基質作用的人造基質混合溶液進行兩點定標，其結果與酶法測定具有良好的可比性。
方法： 分光光度計法
結果：在Jaffe法測定中，常存在血清或血漿干擾物，一般通過減去一個固定常量的肌酐值以消除干擾。但是，在兒童檢測中，使用本法處理數據會出現過度補償的現象。利用一對由參比方法（同位素稀釋質譜法）測定確定的初級血清標準品或者與初級標準品相關的一對二級標準品進行定標，可以使Jaffe法的試驗結果與酶法具有良好的一致性。本定標法存在的問題：由于其他非肌酐干擾性色原出現在標準品中而使定標曲線存在吸光度削減；另外，在兩點定標中，如果一個標準品中含有生理鹽水或水，常會使定標曲線不平滑且存在扭曲。我們準備了一種可以補償Jaffe法測定時血清標準品中干擾色原的人造血清基質并進行了測定。結果表明，即使僅使用一種含有人造基質的標準品進行標定，也可以獲得兩點法標定的結果，并且該結果與使用一對初級標準品定標的結果一致。
結論： 僅應用一種含標準基質并含有肌酐的血清標準品對Jaffe法進行兩點定標，定標后，該法測定血清肌酐的結果和酶法結果具有良好的可比性。酶法是與參考性方法相關的標準方法。
關鍵詞： Jaffe法中用于定標的人造血清基質、Jaffe發中血清干擾原補償物、Jaffe法血清肌酐
文摘鏈接：http://www.reference-global.com/doi/abs/10.1515/CCLM.2008.224
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原文:
Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems
Abstract
Background: The calibration of Jaffe method for serum creatinine using one serum-based standard complemented with artificial matrix compensating standard's Jaffe-interfering substances allows two-point calibration with results well comparable with enzymatic methods.
Method: Spectrophotometry was used.
Results: Jaffe procedures compensating serum/plasma intereferents by subtracting a constant amount of creatinine poorly overcompensate creatinine in children. Two-point calibration with a pair of primary serum standards certified by the reference measurement procedure (isotope-dilution, mass spectrometry) or with a pair of secondary standards linked to primary materials could provide results well agreeable with enzymatic determination. Such a calibration comprises an absorbance offset corresponding to other-than-creatinine Jaffe-interfering chromogens present in standards in the calibration line, while a two-point calibration combining one standard with physiological saline/water always grossly distorts calibration line. We calculated/prepared artificial serum matrices capable of compensating Jaffe-interfering chromogens in serum standards. The combination of even one standard with its artificial matrix also enables two-point calibration with practically the same results as with a pair of primary standards.
Conclusions: A two-point calibration of Jaffe method for serum creatinine combining only one serum standard with creatinine solution matching standard's allow matrix-present interferents present results well comparable with enzymatic determination, providing the standard is attested/linked to the reference measurement procedure.
Clin Chem Lab Med 2008;46:1127–33.
cal Chemistry and Laboratory Medicin