Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab)
Recycling Tubulin (Mitchison Lab)
Labeling Tubulin and Quantifying Labeling Stoichiometry (Mitchison Lab)
Tubulin Polymerization with GTP/GMPCPP/Taxol (Mitchison Lab)
Preparation of Segmented and Polarity Marked Microtubules (Mitchison Lab)
Microtubule Spindowns for Visual Analysis (Mitchison Lab)
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC(Mitchison Lab)
Negative Stain Electron Microscopy of Microtubules (Mitchison Lab)
Microtubule Pelleting (Mitchison Lab)
Actin - Chromatography Purification (The Cell Biology and Cytoskeleton Group, HMS)
Actin - Labeling with Pyrene Iodoacetamide (The Cell Biology and Cytoskeleton Group, HMS)
Actin Binding Assay with Rhodamine-Phalloidin (The Cell Biology and Cytoskeleton Group, HMS)
Actin Extraction from Acetone Powder (The Cell Biology and Cytoskeleton Group, HMS)
Actin Preparation - Acetone Powder Method (The Cell Biology and Cytoskeleton Group, HMS)
Plasma Gelsolin Preparation (The Cell Biology and Cytoskeleton Group, HMS)
Some Buffers for Actin Work (The Cell Biology and Cytoskeleton Group, HMS)
Fixing and Immunostaining Cells (The Cell Biology and Cytoskeleton Group, HMS)
Fixing and Staining Cells for Actin with Fluorescently-Labeled Phalloidin (The Cell Biology and Cytoskeleton Group, HMS)
"Cells on Gels" (The Cell Biology and Cytoskeleton Group, HMS)
this protocol describes method for culture cell on a thin polyacrylamide-based, collagen-coated flexible substrate. By maintaining a constant total concentration of acrylamide while usr/localying the concentration of bis-acrylamide, it's able to obtain a series of chemically identical substrates with a wide range of flexibility. By using imaging techniques, cells' response to differences in substrate flexibility can be detected.
Immunofluorescent Localization of Tubulin (William H. Heidcamp)
For cultured cell
Isolation of Microtubules (Bovine Brain) (William H. Heidcamp)
Viscosity & Polymerization of Microtubules (William H. Heidcamp)
TEM Visualization of Microtubules (William H. Heidcamp)
SDS Gel Electrophoresis of Tubulin\MAPs(William H. Heidcamp)
Isolation of Actin and Myosin Filaments (William H. Heidcamp)
Yeast Actin Capture Assay (D. Amberg)