Gene Targeting Outline (University of Michigan Transgenic Animal Model Core)
This is a brief outline of the steps necessary to produce mice with a mutation targeted to a specific gene. These animals are referred to as "knock-out" mice or "gene targeted" mice
C. Elegans Gene Knockout (Michael Koelle)
The gene knockout strategy involves using a random mutagen (trimethylpsoralen) to mutagenize a very large number of worms. The worms are divided into many small subcultures and allowed to have progeny. A portion of each subculture is stored alive in the freezer, and genomic DNA is made...
Electroporation of ES Cells and Isolation of H/R Clones #2 (PMCI)