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The phosphorylation of myosin affects its role in smooth muscle contraction, platelet formation and possibly other processes. Phosphorylation by myosin light chain kinase (MLCK) increases myosin activity and dephosphorylation by myosin phosphatase decreases myosin activity. CPI, a factor that binds to and inhibits myosin phosphatase, is a target of phosphorylation by PKC and PKN. The inhibitory activity of CPI is reg......閱讀全文
Four different members of the Sprouty protein family block the cellular proliferation and differentiation induced by several different growth factors,
生物通報道：來自中科院上海生命科學院/上海交通大學醫學院健康科學研究所，同濟大學醫學院的研究人員發表文章，證明了一種腸道細菌中的重要蛋白：Tir能與宿主細胞相互作用，抑制宿主細胞的免疫作用，從而造成病原感染。這項研究揭示了一種前所未有天然免疫應答逃逸機制，相關成果公布在Nature
實驗概要The following procedure provides a method of detection of phosphorylated proteins.實驗步驟1. To a sample of protein solution containing 1-100 n
Cdk5 is a cyclin dependent protein kinase involved in dopaminergic signaling in the neostriatal region of the brain. The role of cdk5 in dopamine resp
Cyclin-dependent kinase 5 (cdk5), a multi-functional kinase, and its neuron-specific activator p35 are required for neurite outgrowth and cortical lam
Progression through the cell cycle is accompanied by activation of the proto-oncogene c-Src, a protein tyrosine kinase. Overexpression of Src leads to
The defects in cAMP-regulated chloride channel CTFR are believed to be the major cause for cystic fibrosis. Regulation of CFTR protein by the surface
實驗概要 Small synthetic peptide substrates are especially well suited for applications such as assays of tyrosine kinases
The calcium/calmodulin-dependent kinases (CaMKs) are involved in a large number of cellular responses induced by hormones, neurotransmitters and other
The chromatin packaging of the genome is dynamic, changing with the cell cycle and with transcriptional regulation. During mitosis, chromatin is conde
Mitogen-activated protein (MAP) kinases are important players in signal transduction pathways activated by a range of stimuli and mediate a number of
PTEN is a tumor suppressor gene. Recombinant PTEN is capable of dephosphorylating phosphatidylinositol 3,4,5-triphosphate[PI(3,4,5)P3], the product of
實驗概要The method provides a protocol for removal of phosphate groups from proteins, before or after blotting. To demonstrate the specificity
Although insulin and glucagon play important roles in regulating the response of cells to nutrients, cells also respond to carbohydrates through trans
Monoclonal Antibodies Specific for Proteins in the Oxidative Phosphorylation SystemOxidative phosphorylation (OxPhos) activity occurs in the mitochond
Nitric oxide (NO) has a number of important physiological actions in the cardiovascular system. In the heart, NO plays role in keeping the vessels pat
The signal transduction cascade responsible for sensing light in vertebrates is one of the best studied signal transduction processes, and is initiate
The differentiation of keratinocytes constantly replenishes the upper layers of human skin we lose each day. One factor that contributes to terminal k
The role of Hemophilus influenzae in ear infections and chronic obstructive pulmonary disease includes the induction of an inflammatory response throu
Liver is the major site for carbohydrate metabolism (glycolysis and glycogen synthesis) and triglyceride synthesis (lipogenesis). While insulin was lo
GST Fusion Protein Purification from Yeast5 ml overnight culture of your favorite yeast in your favorite medium.Inoculate 50 ml and grow 30o C shaking
The differentiation of muscle cells is regulated by many factors, including the MyoD/MEF2 family of transcription factors. The MyoD/MEF2 dimer binds t
NK (natural killer) cells are lymphocytes distinct from B and T cells that induce perforin-mediated lysis of tumor cells and virus-infected cells. NK
Interaction of T cell receptor with specific antigen in the context of MHC II activates a signal transduction pathway that leads to T cell activation.
Osteoblasts mineralize bone matrix by promoting hydroxyapatite crystal formation and growth in the interior of membrane-limited matrix vesicles (MVs)
MaterialsRIPA buffer (RIPA buffer enables the extraction of cytoplasmic, membrane and nuclear proteins and is compatible with many applications, inclu
PTEN (gene of phosphates and tensin homologue deleted on chromosome ten)是腫瘤抑制基因，其編碼的具有磷酸酯酶活性的蛋白，是機體維持正常生理功能所必須的因子。作為腫瘤抑制基因，PTEN對受體酪氨酸激酶(receptor tyr
The hepatocyte growth factor receptor, also called c-Met, is activated by HGF and stimulates proliferation of hepatocytes and other cell types. Mutate
Many key signaling molecules are conserved from yeast to man. mTOR is a protein kinase involved in nutrient and growth factor signaling in humans that
Heart formation is cued by a combination of positive and negative signals from surrounding tissues. Inhibitory signals that block heart formation in a