AlexaFluor?488AnnexinV／DeadCellApoptosisKit
實驗概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apoptosis is implicated in disease states, such as Alzheimer’s disease and cancer. Apoptosis is distinguished from necrosis, or accidental cell death, by characteristic morphological and biochemical changes, including compaction and fragmentation of th......閱讀全文
Apoptosis: Miniassay
1) Aliquot 5 X 106 total cells in 5-8 ml 2% media. 2) Add 30 μl 3H-thymidine. 3) Incubate for approximately 16 hours under normal growth conditions
Apoptosis Induction
IntroductionWhen studying induction of apoptosis via a cell surface molecule, it is important to first ascertain surface expression of the molecule of
Caspase Cascade in Apoptosis
Apoptosis, programmed cell death, is triggered by a variety of stimuli, including cell surface receptors like FAS, mitochondrial response to stress, a
Chemical Induction of Apoptosis
Chemical Induction of Apoptosis - 1 May 2001p53, p21WAF1, Myc, Bcl-2, Bax, Bcl-x and bak are among the proteins involved in the regulation of apoptosi
Free Radical Induced Apoptosis
Oxidative stress is one factor that can trigger programmed cell death. Activated neutrophils responding to inflammatory stimulation produce reactive o
DNA Fragmentation Assays for Apoptosis
Protocol I:?Triton X-100 Lysis BufferIn 96 flat-wells plate, incubate 4x10 6 target cells (40 wells of 105 per well) with desired concentration of eff
Granzyme A mediated Apoptosis Pathway
One mechanism used by cytotoxic T cells to kill tumor cells and virus-infected cells is the release of perforin and granzyme proteins. Perforin protei
FACS Procedures for Apoptosis Detection
Materials:Hoechst?33258?(Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500μg/ml (50μl stock + 950μl PBS).7-Amino-actinomycin (Sigma A-94
Apoptosis TUNEL assay (Paraffin Sections)
Protocol for Paraffin Sections: Dewax paraffin sections: Incubate slides, 55°C, 30 min. Xylenes, 2 times, 2 min. each 100% EtOH, 2 times, 2 min.
Guide to Cell Proliferation and Apoptosis Methods
Chapter 1: Cell Death - Apoptosis and Necrosis 1.1 Introduction 2 1.1.1 Terminology of cell death 2 1.1.2 Differences between necros