apipelinefortheidentificationofintactNglycopeptides（一）
pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCDand CID-MS/MS and MS3 Wen-Feng Zeng1,2,*, Ming-Qi Liu3,*, Yang Zhang3,*, Jian-Qiang Wu1,2,*, Pan Fang3,Chao Peng4, Aiying Nie5, Guoquan Yan3, Weiqian Cao3, Chao Confident characterization of the microheterogeneity of protein glycosylation through identification of intact glycopeptides remains one of the toughest analytical chall......閱讀全文
a－pipeline－for－the－identification－of－intact－Nglycopeptides（一）
pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCDand CID-MS/MS and MS3?Wen-Feng Zeng1,2,*, Ming-Qi Liu3,*, Yang Zhang3,
a－pipeline－for－the－identification－of－intact－Nglycopeptides（二）
Methods?Materials. A uniform mixture of six standard glycoproteins was used as the starting material, including IgG (56834, catalog numeber), IgA
a－pipeline－for－the－identification－of－intact－Nglycopeptides（六）
??Figure 4. Comparison of different decoy strategies. The estimated FDR of each decoy method is compared with the real FDR. “Decoy: + 11” means in
a－pipeline－for－the－identification－of－intact－Nglycopeptides（四）
Interpretation of MS3 data. MS3 data were converted to “.ms3” format by pXtract within pFind Studio (version 2.8)26,27, and then analyzed by pFind
a－pipeline－for－the－identification－of－intact－Nglycopeptides（三）
Figure 1. The overall workflow of pGlyco. First the sample is analyzed by HCD-MS/MS (NCE = 40%).Then the product-dependent CID-MS/MS and data-depend
a－pipeline－for－the－identification－of－intact－Nglycopeptides（七）
Complementary ion information provided by HCD- and CID-MS/MS. Both HCD- and CID-MS/MScould be used to optimize the glycopeptide identification. Rece
a－pipeline－for－the－identification－of－intact－Nglycopeptides（五）
Alignment between MS/MS and MS3 identifications. Glycans were identified after analyzing the HCD/CID-MS/MS spectrum pairs, but the sequences of pe
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites四
Figure 2 Distribution of N-glycopeptides analyzed by different enriched methods and instruments of royal jelly proteins. A is the distribution of N-
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites一
Comprehensive identification of novel proteins and N-glycosylation sites in royal jellyLan Zhang1,2?, Bin Han1?, Rongli Li1, Xiaoshan Lu1,3, Aiying Ni
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
CarbohydrateSpecific－Adhesion－of－Intact－Cells－to－Resolved－Glycolipids－on－T
Carbohydrate-Specific Adhesion of Intact ???? Cells to Resolved Glycolipids on TLC PlatesRonald L. Schnaar~Professor, Johns Hopkins University Medical
Identification－and－expansion－of－the－tumorigenic－lung－cancer－stem－cell－...
Identification and expansion of the tumorigenic lung cancer stem cell populationLung cancer contains a rare population of CD133+ cancer stem-like ce
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
A－Yeast－Secretion－Trap－Assay－for－Identification－of－Secreted－Proteins－...
Secreted proteins from plants and phytopathogens play important roles in their interactions and contribute to elaborate mechanisms of attack, defe
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（三）
?Target analysis by Parallel reaction monitoring (PRM)The targeted quantification and verification were carried out in 94 serum samples with 5~6 mic
Comparative－assessment－of－glycosylation－of－recombinant－human－...（一）
Comparative assessment of glycosylation of recombinant human FSH and highly purified FSHHong Wang, Xi Chen, Xiaoxi Zhang, Wei Zhang, Yan Li, Hongrui Y
Identification－of－a－Mutant－Kinase/ATP－Analog－Pair1
Identification of a Mutant Kinase/ATP Analog PairScott T. Eblen, N. Vinay Kumar, and Michael J. WeberDepartment of Microbiology and Cancer Center, Uni
Identification－of－a－Mutant－Kinase/ATP－Analog－Pair2
Cellular Transfection and Immunoprecipitation??Before proceeding with the experiments outlined below, all kinase pocket mutants should be characterize
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites二
ResultsIdentified novel royal jelly proteins ?To expand the number of known proteins in the RJ proteome, RJ proteins were extracted and digested wit
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites三
?Note: All of the identified proteins are from Apis mellifera. Accession is the unique number given to mark the entry of a protein in the database
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites七
Data analysisTandem mass spectra were retrieved using Xcalibur (version 2.2, Thermo Fisher Scientific) and AnalystTF (version 1.6, AB SCIEX) softw
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites八
References1. Fujita T, Kozuka-Hata H, Ao-Kondo H, Kunieda T, Oyama M, Kubo T: Proteomic analysis of the royal jelly and characterization of the fu
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites九
41. Schmidt O, Theopold U, Strand M: Innate immunity and its evasion and suppression by hymenopteran endoparasitoids. BioEssays 2001, 23(4):344–35
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites六
N-glycosylation modification of proteins has reported to improve the health of living organisms through antibacterial activity [68], antioxidant a
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites五
RJ provides efficient energetic fuels for the fast development of larvae and the egg-laying queen through the metabolism of sugars, lipids, and pr
GenomeWide－Identification－of－Transcription－FactorBinding－Sites－in...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip)
Pipeline血流導向裝置治療顱內大型動脈瘤診療分析
隨著神經介入技術和器械的發展，中小型顱內動脈瘤的介入治療效果確切，而大型或巨大型動脈瘤的介入治療效果不滿意，其中重要的原因是動脈瘤復發。近年來，隨著載瘤動脈重建概念的提出，一種新型的血流導向裝置Pipleline應運而生。有研究表明，血流導向裝置主要用于治療未破裂的寬頸動脈瘤、梭型動脈瘤及大型或巨大
血流導向裝置Pipeline治療以單側眼瞼下垂為首發表現的...
血流導向裝置Pipeline治療以單側眼瞼下垂為首發表現的頸內動脈巨大動脈瘤病例分析Pipeline血流導向裝置（Pipeline?embolization?device，PED）主要用于血管腔內治療成人頸內動脈巖骨段至垂體上動脈開口處近端的大型或巨大寬頸動脈瘤，對于超適應證范圍的，如后循環動脈瘤、
Pipeline血流導向裝置治療大腦中動脈復雜動脈瘤的初步...
Pipeline血流導向裝置治療大腦中動脈復雜動脈瘤的初步經驗大腦中動脈瘤是顱內動脈瘤的常見類型，采用開顱夾閉及彈簧圈栓塞（或支架輔助）多可獲得滿意療效。對于大腦中動脈復雜動脈瘤，包括大型、巨大型、梭形、復發、寬頸動脈瘤及瘤頸鈣化、動脈瘤或瘤頸有重要分支血管發出的動脈瘤等，采用支架輔助下彈簧圈栓塞難
Comparative－assessment－of－glycosylation－of－recombinant－human－...（二）
Experimental sectionChemicals and reagentsOne lot of PuregonR-HP of 50 IU/0.5mL and two lots of PuregonR-HP of 100 IU/0.5mL (rhFSH) (Organon, Oss, N