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1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M Tris, pH 9-11(8) PBS2. Procedure(1) Dilute Ab 1:5 with Binding Buffer. Filter through a 0.22 m filter.(2) Equilibrate column with at least 2 bed volumes of Binding Buffer(3)......閱讀全文
NM_002128 Homo sapiens high-mobility group box 1 (HMGB1
NM_033142 Homo sapiens chorionic gonadotropin, beta pol
Preparation of Glutathione-S-Transferase (GST) Fusion ProteinsMargret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia,
An Introduction to BiocomputingVersion 2.01October 1996David Steffen, Ph.D.President, Biomedical Computing, Inc.6626 WestchesterHouston, Texas 77
以下是所有的看家基因的列表，大家在設計引物時，可以直接采用這些基因的序列，本表來自于Trends in Gentics上一篇文獻，內容全面，所有的看家基因均標注了基因銀行號（Genbank），可以直接到pubmed中查詢得出。引物設計可以推薦用primer 5，也可以直接與我們聯系設計，有關事宜，請
Background:Proteins, like many molecules, can be prompted to form crystals when placed in the appropriate conditions. In order to crystallize a protei
The sedimentation coefficient of a protein is a measure of how fast it moves through the gradient. Increasing the mass of the protein will increase it
1、Required Materials and Equipment(1) Protein A or G agarose gel column (10 ml or 5 ml of packed beads; see guidelines below for choice of protein A o
Determining the Molecular Weight of a Protein Molecule—Combining S and R s à la Siegel and MonteWith the completion of multip
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
蛋白質提取和純化（主要內容如下）Protein Extraction Protein PurificationProtein PrecipitationColumn PreparatioinQ & A Posted in the Method ForumProtein Extrac
Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Correspon
生物分子發表的代表性文獻 QTRAP：同時具有三重四極桿和線性離子阱性能的獨一無二的LC/MS/MS系統 QTRAP系統最早在ASMS 2002上，作為第一臺商用的線性離子阱發布，是世界上唯一的線性離子阱和三重四極桿的復合
Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron MicroscopyAn important part of ch
Lowry – Protein Determination(From Protein Protocols on CD-ROM Humana Press, 1998 - Section 1-2 The Lowry Method for Protein Quantitation Jakob H. Wat
實驗概要Protein G, a cell wall component produced by group G Streptococcus strains, binds the Fc part of a wide range of immunoglobulins (Ig’s
表1 蛋白質相互作用分析相關數據庫及網站 網站 資源類型 網址 DIP 蛋白質相互作用http: //dip.doe-mbi.uda.edu INTERACT 蛋白質相互作用http: //bioinf.man.ac.uk/interact
實驗概要Preparation of lysis buffers, protease and phosphatase inhibitors, lysate from cell culture, lysate from tissues, protein concentratio
Quantitative Determination of Peptides by Sulfhydryl (-SH) Groups New (Contributed by David Van Horn, Dept. of Chemistry, UC Berkeley Greg B
近紅外分析儀在飼料品質分析的應用場景 近紅外分析儀在配合飼料整個生產過程中可以設置的質量監控點，如圖1所示的1~5。 1）原料進廠時的品質監控 2）生產工藝過程及時監控：在混合工藝、膨化工藝及制粒工藝設置品質監控點 3）成品質量判斷 圖1：配合飼料生產流程圖近紅外分析儀在飼料品質分
參考文獻：1． Morrow DA, Ridker PM. C-reactive protein, inflammation, and coronary risk. Med Clin North Am, 2000,84:149-161.2． Hashimoto H, Kitagawa K, Houg
借助Chromotek公司GFP-Trap如何設計成功的IP實驗？How to plan an immunoprecipitation of your GFP-fusion protein when using the ChromoTek GFP-Trap?PreambleThis document
Motor proteins of several kinesin family groups have now been crystallized: monomeric Kinesin-1 motor domains from human, rat andNeurospora (Kull
The Kirkwood/Bloomfield CalculationThe understanding of how protein shape affects hydrodynamics is elegantly extended by an analysis originally develo
摘要：基于抗體的蛋白質檢測方法，主要有western blot、ELISA、點雜交以及免疫組化等，這些方法被廣泛地應用于科研和診斷領域。在蛋白質的免疫檢測過程中，樣品蛋白首先結合與特異性一抗上，然后再用攜帶標簽（諸如：熒光染料，放射性元素，酶等）的二抗進行檢測。然而，為了避免種間內的交叉反應，必
3. ImmunoprecipitationImmunoprecipitation can be performed using antibodies by different methods. The use of these methods is based on the requi
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
（一）細胞計數 1、將血球計數板及蓋片用擦試干凈，并將蓋片蓋在計數板上。 2、將細胞培養基懸液吸出少許，滴加在蓋片邊緣，使懸液充滿蓋片和計數板之間。 3、靜置3分鐘。 4、鏡下觀察，計算計數板四大格細胞總數，壓線細胞只計左側和上方的。然后按下式計算： 細胞數/ml＝4大格細胞總數/ 4×1
Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly
COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODSCompanyMethodDetection RangeApplications -CompatibilityAssay protocolPrecautions