Angiotensinconvertingenzyme2regulatesheartfunction
Cardiovascular diseases are predicted to be the most common cause of death worldwide by 2020. The major regulator of blood pressure homeostasis is the renin-angiotensis system. Angiotensinogen is digested by renin to produce angiotensin I (AGT I). AGT I is an inactive 10 amino-acid peptide that is further degraded to produce angiotensin II by the agiotensin-converting enzyme (ACE). Angiotensis II is the master regula......閱讀全文
Angiotensinconverting－enzyme－2－regulates－heart－function
Cardiovascular diseases are predicted to be the most common cause of death worldwide by 2020. The major regulator of blood pressure homeostasis is the
NFAT－and－Hypertrophy－of－the－heart－(Transcription－in－the－broken－heart)
Hypertrophy associated with both hypertension and obstruction to ventricular outflow leads to pathologic cardiac growth and it is associated with incr
Regulation－And－Function－Of－ChREBP－in－Liver
Liver is the major site for carbohydrate metabolism (glycolysis and glycogen synthesis) and triglyceride synthesis (lipogenesis). While insulin was lo
In－VitroCulture－of－Chicken－Heart
The chicken is a classic organism used to illustrate the principles of basic embryology. One of the developmental systems which has been examined in g
Mitochondria－Structure－and－Function－－Theoretical－Details
Mitochondria Structure and Function - Theoretical DetailsIn an attempt to be concise and understandable, introductory level courses and textbooks freq
Reverse－Transfection－for－Gene－Function－Analysis
This guide describes a microarray-based system for the functional analysis in mammalian cells of many genes in parallel. Mammalian cells are cultured
Function－of－SLRP－in－Bone:－An－Integrated－View
SLRPs (Small leucine-rich proteoglycans) are extracellular molecules that bind to TGF-betas, collagens and other matrix molecules. In vitro, SLRPs wer
Restriction－Enzyme－Buffer
Restriction Enzyme Buffer?Most enzymes can use REact buffers; however, some are made up separately.?Use fresh Milli-Q water, siliconized or sterile gl
The－Restriction－Enzyme－Database
REBASE?The Restriction Enzyme Database?The?Restriction?Enzyme data?BASE?A collection of information about restriction enzymes and related proteins. It
Actions－of－Nitric－Oxide－in－the－Heart
Nitric oxide (NO) has a number of important physiological actions in the cardiovascular system. In the heart, NO plays role in keeping the vessels pat
whats－the－function－of－compressed－air－filter
Compressed air is an essential utility in many industrial applications, including pneumatic tools, packaging, and conveying systems. It is a clean, ef
Sumoylation－by－RanBP2－Regulates－Transcriptional－Repression
Current data indicates that nuclear pore complex (NPC) RanBP2 (SUMO E3 ligase) protein sumoylates Mdm2 and HDAC proteins during nuclear translocation.
Enzyme－Kinetics－assay－of－the－WT
To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media
Restriction－Enzyme－Digestion－of－DNA
Materials:10X restriction enzyme buffer (see manufacturer's recommendation)DNAsterile waterrestriction enzymephenol:chloroform (1:1)Add the follow
Regulation－of－PGC1a
Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1a) is a tissue-specific coactivator that enhances the activity of many nucl
Neutrophil－and－Its－Surface－Molecules
Neutrophils are important phagocytotic leukocytes (white blood cells) that internalize and destroy infectious bacteria by a respiratory burst of react
ELISPOT－(Enzymelinked－ImmunoSPOT)
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
gSecretase－mediated－ErbB4－Signaling－Pathway
The HER4/erbB4 receptor tyrosine kinase is a member of the EGF1 receptor family. HER4 is a receptor for the neuregulins (NRGs), a family of growth and
GentleMACS/單細胞懸液制備文獻集錦
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? ? ? ? ? ? 種屬 處理組織 文章名稱 human ? ? ? ? ? ? ?
Proteasome－Complex
Attachment of the ubiquitin peptide to proteins targets them for proteolytic degradation by a complex cellular structure, the proteasome. The regulate
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in?Ambystoma mexicanum?embryos. Specifically, we will split the morphogenetic
Sonic－Hedgehog－(SHH)－Receptor－Ptc1－Regulates－cell－cycle
Sonic Hedgehog (Shh) is a secreted protein identified genetically as an important developmental factor. Shh provides a morphogenic signal in the devel
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates
AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of?Helicobacte
Steady－State－ATPase－Assays－Coupled－Enzyme－System
MaterialsTubulin?(>5 mg/mL)100 mM Mg·GTP4 mM Taxol in DMSOPM =100 mM PIPES pH 6.82 mM EGTA1 mM Mg2SO4Motor protein (>95% purity; 15-20 μM)Cuvettes (20
凝乳酶（curdling－enzyme）的提取純化
一、實驗目的通過發酵液中凝乳酶的提取純化，了解酶提取純化的方法，學習凝膠層析法的工作原理和基本操作技術。二、實驗原理凝乳酶是一種酸性蛋白酶，其主要的生物學功能是有限剪切Phe105- Met106連接的κ-酪蛋白，導致牛奶凝結，從而被廣泛應用干酪生產中。有機溶劑對于許多蛋白質（酶）、核酸、多糖和小分
Histochemical－staining－of－sea－urchin－embryos－for－(AP)－enzyme－activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples, tube of?AP substrate buffer?and tu
Fibrinolysis－Pathway
Clot formation and fibrinolysis is a balance of plasmin activation/inhibition and thrombin-thrombomodulin activity that regulates fibrin polymer forma
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates3
Different enzyme assays for ACTase study in H. pyloriACTase properties were studied?in situ?in cell-free extracts to obtain information on enzyme func
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates4
The use of a micro-titre based colorimetric assay provided a third method for the study of ACTase activity, and the most useful for large scale measur