TheEffectsofNiCl2onSpiculeFormation
The Effects of NiCl2on Spicule FormationJessica Ann Billet, Franklin and Marshall, Class of 2000Background and ObjectiveSea urchins exhibit radial holoblastic cleavage, eventually forming a blastula. Shortly after the blastula hatches from the fertilization membrance, the embryo begins gastrulation. Gastrulation begins when the vegetal side of the blastula begins to thicken and flatten. This flat sheet of cells is ca......閱讀全文
The－Effects－of－NiCl2on－Spicule－Formation
The Effects of NiCl2on Spicule FormationJessica Ann Billet, Franklin and Marshall, Class of 2000Background and ObjectiveSea urchins exhibit radial hol
Tube－formation－assay
DescriptionThis is a fast and easy assay to test the angiogenic/anti-angiogenic properties of molecules. As compared to other angiogenesis assays, suc
SOFT－AGAR－ASSAY－FOR－COLONY－FORMATION
Note: All volumes are calculated to cater for four plates per point.Base Agar1. Melt 1% Agar (DNA grade) in microwave, cool to 40 in a waterbath. War
The－Effects－of－Ethanol－on－Zebrafish－Development
Introduction:The early stages of zebrafish,?Danio rerio?development are characterized by meroblastic dicoidal cleavage. Initially cell division only o
Effects－of－calcineurin－in－Keratinocyte－Differentiation
The differentiation of keratinocytes constantly replenishes the upper layers of human skin we lose each day. One factor that contributes to terminal k
The－Effects－of－Ultraviolet－Light－on－the－Fertilization
Jill K. Flemming, Franklin & Marshall College, Class of 2001IntroductionThe objective of this project is to observe the effects of UV radiation on bot
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
The－Developmental－Effects－of－Nicotine－on－Chick－Embryos
More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette
Determining－the－effects－of－nicotine－on－bone－－in－chicks
ObjectiveThe purpose of this experiment is to determine how the development of bone and cartilage in a chick are affected by treating the embryo with
Joint－formation－in－chick－limb－bud－CAM－grafts
Abstract????????Choriallantoic membrane (CAM) limb grafting functions as a method to isolate the inductive events of limb formation. Experimenters hav
Formation－of－Ketone－Bodies－from－acetylCoA
The acetyl-CoA produced by mitochondrial beta-oxidation of fatty acids enters the Kreb's cycle to produce energy, but that is not the only fate of
DEVELOPMENTAL－EFFECTS－OF－LITHIUM－CHLORIDE－ON－XENOPUS－EMBRYOS
PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula?xenopus?embryos. INTRODUCTION: When
The－effects－ofdifferent－concentrations－oflithium－chloride－on－thedevelopment
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular?Brachydanio rerio?embryos results in
Histochemical－staining－of－sea－urchin－embryos－for－(AP)－enzyme－activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples, tube of?AP substrate buffer?and tu
Basic－mechanism－of－action－of－PPARa,－PPARb(d)－and－PPARg－and－effects
Similar to other nuclear hormone receptores, PPAR acts as a ligand activated transcription factor. Upon binding fatty acids or hypolipidemic drugs, PP
Polyphenoloxidase－(catechol－oxidases)－assay
Browning of the cut surface of some fruits and vegetables is due the presence of a group of enzymes called polyphenoloxidases. These enzymes are relea
The－Effects－of－CalciumFree－Seawater－onthe－Development－ofSea－Urchin－Embryos
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
RhoSelective－Guanine－Exchange－FactorAKAP13－Mediates－Stress－Fiber－Formation
The A-kinase anchor protein 13 (AKAP13, also known as AKAP-LBC) is one of a group of structurally diverse proteins, which have the common function of
Effect－of－UV－Treatment－on－Early－Development－of－Sea－Urchin－Embryos
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
Cytokine－induced－angiogenesis－in－chick－embryos.
Objective:The purpose of this experiment was to explore the effects of the growth factors bFGF and VEGF on blood vessel formation within the chorioall
Dynamic－Monitoring－ofCellular－Remodeling－Induced－bythe－Transforming－Growth2
Results and discussionData acquisition demonstrated a linear increasing of the CI values in control cells during the time interval observed. However,
Dynamic－Monitoring－ofCellular－Remodeling－Induced－bythe－Transforming－Growth2
Results and discussionData acquisition demonstrated a linear increasing of the CI values in control cells during the time interval observed. However,
SingleCell－Electroporation－in－Xenopus
Single-Cell Electroporation in Xenopus Xue Feng Liu and Kurt Haas INTRODUCTION Single-cell electroporation (SCE) is a versatile technique for del
Metabolism－of－Anandamide,－an－Endogenous－Cannabinoid
The lipid anandamide derived from arachidonic acid binds to the cannabinoid G-protein coupled receptors CB-1 and CB-2 to exert its biological effects.
How－Progesterone－Initiates－Oocyte－Membrane
Progesterone (Pg) binds to both intracellular iPR and plasma membrane- bound mPR. (Right Top) After binding to Pg, iPR is recruited to the membrane as
Role－of－nicotinic－acetylcholine－receptors－in－the－regulation－of－apoptosis
Nicotinic acetylcholine receptors are essential for neuromuscular signaling and are also expressed in non-neuronal tissues, where their function is le
The－effect－of－lithium－chloride－on－Lytechinus－variegates－embryos
PurposeThis experiment will study effects of lithium chloride on sea urchin development, forcusing on archenteron formation.IntroductionGastrulation i
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates3
Different enzyme assays for ACTase study in H. pylori ACTase properties were studied?in situ?in cell-free extracts to obtain information on enzyme f
Ion－Channels－and－Their－Functional－Role－in－Vascular－Endothelium
Endothelial cells (EC) form a multifunctional signal-transducing surface that performs different tasks dependent on its localization