The use of a micro-titre based colorimetric assay provided a third method for the study of ACTase activity, and the most useful for large scale measurements. The method was essentially as described previously (9), but several findings proved useful in optimising the assay for this work. Although the authors suggest that the colour development reagents can be stored for a short period of time (10), it was found in the......閱讀全文
Different enzyme assays for ACTase study in H. pyloriACTase properties were studied in situ in cell-free extracts to obtain information on e
AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of Helico