GramStain(+\)
實驗概要細菌的革蘭氏染色技術實驗材料Colonies of bacteriaToothpicksCrystal violetGram's iodine95% ethanolSafraninMicroscopes with oil immersion實驗步驟1. Before staining the individual colonies, you should first practice the technique by observation of the gram positive micro- organisms normally found in the gum linings of your mouth.2. Use a clean toothpick to rub along the gingival crevices (area between tooth surface and gums)......閱讀全文
Gram－Stain－(+\)
實驗概要細菌的革蘭氏染色技術實驗材料Colonies of bacteriaToothpicksCrystal violetGram's iodine95% ethanolSafraninMicroscopes with oil immersion實驗步驟1. Before staini
Gram－Stain－(+\)
MaterialsColonies of bacteria from Exercise 12.2ToothpicksCrystal violetGram''s iodine95 ethanolSafraninMicroscopes with oil immersionProcedur
細菌的簡單染色(Simple－stain)與革蘭氏染色(Gram－stain)
一、目的要求1、學習細菌染色的原理和方法；2、掌握細菌的簡單染色法和革蘭氏染色法。二、基本原理用于生物染色的染料主要有堿性染料、酸性染料和中性染料三大類。堿性染料的離子帶正電荷，能和帶負電荷的物質結合。因細菌蛋白質等電點較低，當它生長于中性、堿性或弱酸性的溶液中時常帶負電荷，所以通常采用堿性染料（如
Testing－for－Mycoplasma－by－Indirect－DNA－Stain－(Hoechst－33258－stain)
AimDNA staining methods such as Hoechst staining techniques are quick with results available within 24 hours, which compares favorably with 4 weeks fo
Gramstaining－Procedure
Gram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should
Alcian－green－stain－for－cartilage
This is an easy method for stianing cartilage that can be used with isolated or CAM-grafted limbs to study limb development or with whole embryos (8 t
DAPI－Nucleic－Acid－Stain
實驗概要The ?blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it ?appears to associate with AT clusters in the minor groove. Binding
Vybrant?－DyeCycle?－Violet－Stain
實驗概要Live cell studies ?of cellular DNA content and cell cycle distribution are useful to detect ?variations of growth patterns due to a variety of phy
Vybrant?－DyeCycle?－Ruby－stain
實驗概要Live cell studies ?of cellular DNA content and cell cycle distribution are useful to detect ?variations of growth patterns due to a variety of phy
細菌檢測
Gram Staining (+\-)?(William H. Heidcamp)??Gram-Staining Procedure?(MEDIC, U of Texas)Very nice and detailed method description for Gram staining??Aci
SMEAR－PREPARATION
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria
Negative－Stain－Electron－Microscopy－of－Microtubules
Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of
Human－Placental－Alkaline－Phosphatase－Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike othe
Permeabilization－of－gramnegative－bacteria－with－KPi/hexane
Harvest (10,000 rpm for 5 min) 24 h grown (in LB pH 7.5, 37?oC, 200 rpm)?Escherichia coli,?Enterobacter aerogenes?or?Pseudomonas aeruginosa?cells.Wash
革蘭氏(Gram)染色液的配制方法
革蘭氏(Gram)染色液 1．草酸銨結晶紫染液 A液：結晶紫(crystal violet) 2g 95%酒精 20ml B液：草酸銨(ammonium oxalate) 0．8g 蒸餾水 80ml 混合A、B二液，靜置48小時后使用。 2．盧戈氏(Lugol)碘液 碘片 1．0g
細菌的鞭毛染色(Flagella－stain)
實驗器材1.活材料培養12-16h的水稻黃單胞菌（Xanthomonas oryzae），粘質賽氏桿菌（Serratia marcescens）或假單細胞菌（Pseudomonas sp.）斜面菌種。2.染色液和試劑硝酸銀染色液、Leifson染色液、香柏油、二甲苯。3.器材載玻片、擦鏡紙、吸水紙、
細菌的莢膜染色(capsule－stain)
實驗原理由于莢膜與染料間的親和力弱，不易著色，通常采用負染色法染莢膜，即設法使菌體和背景著色而莢膜不著色，從而使莢膜在菌體周圍呈一透明圈。由于莢膜的含水量在90%以上，故染色時一般不加熱固定，以免莢膜皺縮變形。 實驗器材 1.活材料 培養3-5天的膠質芽孢桿菌（Bacillus muci
Chromosomal－DNA－Extraction－from－Grampositive－Bacteria
Chromosomal DNA Extraction from Gram-positive BacteriaThis procedure was originally developed for?Listeria monocytogenes?but has worked well with othe
組織學一般技術
The manuals for this section have been compiled by Susan L. Wall, HT(ASCP). These manuals are in Adobe Acrobat PDF format and require the Acrobat Read
LIVE/DEAD?－Fixable－Dead－Cell－Stain－Kits
實驗概要The LIVE/DEAD? ?Fixable Dead Cell Stain Kits use a novel method to evaluate the ?viability of mammalian cells by flow cytometry. These assays are
LIVE/DEAD?－Fixable－Dead－Cell－Stain－Kits
實驗概要The ?LIVE/DEAD? Fixable Dead Cell Stain Kits use a novel method to evaluate ?the viability of mammalian cells by flow cytometry. These assays are
革蘭氏染色檢查正常參考值及臨床意義
中文名稱：革蘭氏染色檢查 英文名稱：Gram stain Examination 臨床意義： 胸腹水、分泌物等涂片革蘭氏染色檢出細菌，可考慮為細菌感染。 對培養基生長細菌進行染色分類，并觀察形態。
Microscopy－with－Oil－Immersion
Microscopy with Oil ImmersionPrincipleWhen light passes from a material of one refractive index to material of another, as from glass to air or from a
細菌感染的微生物學檢查方法
醫生根據臨床上診斷和治療的需要選擇不同的標本和檢查方法進行實驗室診斷，從而鑒定出感染的病原菌。實驗室診斷包括細菌學診斷，即檢測及鑒定病原菌；檢測病原菌成分（抗原和核酸）以及檢測患者血清中特異性抗體（圖1）。圖1 細菌感染的實驗診斷方法??一、細菌學診斷??形態學檢查 光鏡下可觀察直接涂片或分離培養的
革蘭氏染色與細菌抗酸染色技術的操作技巧
蘭氏染色和抗酸染色都屬于細菌形態學檢查法，提到革蘭氏染色大家肯定都非常熟悉，但是說到抗酸染色你不一定了解。今天我們進入細菌的花花世界，聊一聊細菌界的這兩大染色法：??革蘭氏染色（Gram stain）?用途：由丹麥病理學家Christain Gram于1884年創立，直到現今，革蘭氏染色法仍是細菌學
革蘭氏染色反應的基本內容
革蘭氏染色反應是細菌分類和鑒定的重要性狀。它是1884年由丹麥醫師Gram創立的。革蘭氏染色法(Gram stain)不僅能觀察到細菌的形態而且還可將所有細菌區分為兩大類：染色反應呈藍紫色的稱為革蘭氏陽性細菌，用G+表示；染色反應呈紅色(復染顏色)的稱為革蘭氏陰性細菌，用G-表示。 細菌對于革
革蘭氏染色反應的相關介紹
革蘭氏染色反應是細菌分類和鑒定的重要性狀。它是1884年由丹麥醫師Gram創立的。革蘭氏染色法（Gram stain）不僅能觀察到細菌的形態而且還可將所有細菌區分為兩大類：染色反應呈藍紫色的稱為革蘭氏陽性細菌，用G+表示；染色反應呈紅色(復染顏色)的稱為革蘭氏陰性細菌，用G-表示。 細菌對于革
細菌的簡單染色（Simple－stain）和革蘭氏染色2
（3）固定：手執玻片一端，讓菌膜朝上，通過火焰2-3次固定（以不燙手為宜），使細胞質凝固，以固定細菌的形態，并使其不易脫落。但不能在火焰上烤，否則細菌形態將毀壞（4）染色：將固定過的涂片放在廢液缸上的擱架上，加復紅染色1-2min（5）水洗：用水洗去涂片上的染色液，直至沖下之水無色時為止。（6）干燥
細菌的簡單染色（Simple－stain）和革蘭氏染色1
雖然各種類型的顯微鏡能夠觀察到微生物的各種形態結構，但一般實驗室常用的是普通光學顯微鏡。由于細菌體積小且透明，在活體細胞內又含有大量的水分，因此，對光線的吸收和反射與水溶液相差不大。當把細菌懸浮在水滴內，放在顯微鏡下觀察時，由于與周圍背景沒有顯著的明暗差，難于看清它們的形狀，更談不上識別其細微結構。
微生物的形態觀察
一．實驗目的1． 學習在油鏡下觀察微生物 ?的個體形態2． 了解革蘭氏染色法的原理，并熟練掌握其操作步 二．實驗原理微生物的細胞小且透明，在普通光學顯微鏡下不易識別，必須對它們進行染色，使經染色后的菌體與背景形成明顯的色差，從而能更清楚地觀察到其形態和結構。因此，微生物染色技術是觀察微生物形態結