Phosphoaminoacidanalysis:MarkKampssmethod
Phosphoamino acid analysis:Mark Kamps's method1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P.Neither nitrocellulose nor nylon will work!Keep the membrane wet and wrap in Saran wrap.Add radioactive markings for orientation of film laterExpose to film.2. Cut out band of interest, re-wet in m......閱讀全文
Phosphoamino－acid－analysis:Mark－Kampss－method
Phosphoamino acid analysis:Mark Kamps's method1.?Label your protein with 32Pi.?Then subject the protein to SDS polyacrylamide gel electrophoresis
Phosphoamino－acid－analysis－of－nonTCA－precipitable－proteins
Phosphoamino acid analysis of non-TCA precipitable proteins 1. Cut out gel slice, incubate 60 min with 1 ml 5.7 M HCl @ 110oC in 13 x 100 mm screw c
蛋白質檢測
·?????????Protein detection?(Aberdeen's Lab)The method used to locate the proteins following 2D-PAGE depends on the nature of the original sample.
蛋白質磷酸化
Tyrosine Kinase Assay Using Synthetic Peptides?(T. Miller)Small synthetic peptide substrates are especially well suited for applications such as assay
磷酸氨基酸分析的方法特點和功能
中文名稱磷酸氨基酸分析英文名稱phosphoamino acid analysis定 義一種分析蛋白質中是否含羥基氨基酸(如絲氨酸、蘇氨酸、酪氨酸等)-O-磷酸酯的方法。應用學科生物化學與分子生物學（一級學科），方法與技術（二級學科）
Neutralizing－Arachidonic－Acid
NOTE - Use arachidonic acid from Biomol (# FA-003) 1) Make up the appropriate concentration of AA in 100% ethanol. 2) Add 1μl of phenol red solutio
Arachidonic－Acid－Labeling
1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum. 2) Pellet cells and wash 1 time with room temperature PBS. 3) Resus
Amino－acid－composition
There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi
The－Citric－Acid－Cycle
The Krebs cycle, also called the citric acid cycle, is a fundamental metabolic pathway involving eight enzymes essential for energy production through
美國FDA擬制定一種新的方法測定食品中亞硫酸鹽
據美國聯邦公報消息，2019年9月17日，美國食品藥品監督管理局（FDA）發布2019-19862號文件，擬制定一種新的方法測定食品中亞硫酸鹽。 據了解，相關意見需在2019年10月17日之前提交。 部分原文報道如下： Our regulations at §§？101.100（a）（4）
Glycosphingolipid－analysis
1) Incubate cells with 1 μCi/ml of 3H-galactose for 72 hours.---> If treatment is for an extended period of time: treat in serum free media containing
Lipid－analysis
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer
DAPI－Nucleic－Acid－Stain
實驗概要The ?blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it ?appears to associate with AT clusters in the minor groove. Binding
Oxidation－of－Polyunsaturated－Fatty－Acid
Unsaturated fatty acids are those that contain one or more double bonds in their alkyl-chain. Polyunsaturated fats with two double bonds usually have
Principles－of－nucleic－acid－hybridization
Principles of nucleic acid hybridization5.2.1.?Nucleic acid hybridization is a method for identifying closely related nucleic acid molecules within tw
Preparation－of－nucleic－acid－probes
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
RNA提取
RNA提取（主要內容如下）Tips for Handing RNA?Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum??Basic Procedures for Handing
Method:－Lymphocyte－Transformation
Method: Lymphocyte Transformation May 30, 1990 Rosalie Veile Principle: Lymphocytes are transformed to establish cell lines. Mononuclear
Rab－GTPases－Mark－Targets－In－The－Endocytotic－Machinery
The eukaryotic cell contains compartments with distinct functions bounded by lipid bilayer membranes. The movement of membrane vesicles between these
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates3
Different enzyme assays for ACTase study in H. pylori ACTase properties were studied?in situ?in cell-free extracts to obtain information on enzyme f
Biosynthesis－and－Analysis－of－Bilins
The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first o
Analysis－of－Heme－and－Hemoproteins
Heme is perhaps the most ubiquitous cofactor found in nature and the most functionally diverse. Hemoproteins are involved in cell respiration (cy
Analysis－of－Oligosaccharide－Ligands
Analysis of Oligosaccharide Ligands?by High Performance Liquid Affinity Chromatography Analysis of Oligosaccharide Ligands by High Performance Liqui
Analysis－of－murine－BM
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis.? Techniques to do this are well established in hu
Lineage－Analysis－of－Blood
Materials:Capillary tubes1.5 mL Eppendorf microfuge tubes15 mL conical centrifuge tubes96-well V-bottom plates (Corning Costar 3894, from Fisher)Flow
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
Flow－Cytometry－Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
Comparative－assessment－of－glycosylation－of－recombinant－human－...（二）
Experimental sectionChemicals and reagentsOne lot of PuregonR-HP of 50 IU/0.5mL and two lots of PuregonR-HP of 100 IU/0.5mL (rhFSH) (Organon, Oss, N
核酸提取(The－Extraction－of－Nucleic－Acid)
【實驗目的】 1．掌握核酸提取的基本原理和基本方法 2．掌握檢測核酸濃度及純度的原理及方法 ? 【實驗原理】 DNA是遺傳信息的載體,是最重要的生物信息分子,是分子生物學研究的主要對象,因此DNA的提取也應是分子生物學實驗技術中最重要、最基本的操作。如不能有效的完成DNA提取方面的工作，那就根本談不
Acid－Phenol－Yeast－RNA－Prep
This is the preferred method for yeast RNA preparationuse Gloves and RNAse free solutions throughout.1. Use a YPD overnight culture to innoculate fres