Amersham2DPAGE中文版

目錄

第一章 樣品制備                                                               2

1.1   一般性原則                                                                  2
1.2   樣品制備程序                                                              3
1.2.1 培養細胞樣品處理方法                                            3
1.2.2  組織樣品處理方法                                                    3
1.2.3 文獻報道較多的裂解液配方                                     4

第二章 第一向等電聚焦 （IEF）                                  7
2.1   IPG 膠條的水化和電泳                                             7
2.1.1 儀器                                                                            7
2.1.2 試劑                                                                            7
2.1.3 實驗步驟                                                                    7
2.2   IPG膠條的平衡                                                           9
2.2.1 儀器                                                                          10
2.2.2 試劑                                                                          10
2.2.3 實驗步驟                                                                  10

第三章 第二向 SDS 電泳                                                12
3.1   垂直 SDS-PAGE                                                     12
3.1.1 溶液                                                                          12
3.1.2 灌膠步驟                                                                  12
3.1.3 電泳步驟                                                                  14

第四章 2-DE 膠蛋白質點的檢測                                     15
4.1   考馬斯亮蘭染色                                                        15
4.1.1 經典的考馬斯亮蘭染色程序                                   15
4.1.2  Neuhoff 膠體考染法                                                15
4.1.3 熱考馬斯亮蘭染色及二次染色法                           16
4.2 硝酸銀染色                                                                  16

Appendix I  Troubleshooting                                            18
Appendix II  Solutions                                                       23