BioradProteinAssay:Bradford

Biorad Protein Assay: Bradford

Standards: 1 mg/ml BSA stock

- dilute 1:10 to get 0.1 mg/ml BSA

Add To get H-2O

20 μl 2 μg/ml 780 μl

40 μl 4 μg/ml 760 μl

60 μl 6 μg/ml 740 μl

80 μl 8 μg/ml 720 μl

100 μl 10 μg/ml 700 μl

120 μl 12 μg/ml 680 μl

Protocol:

1) Add 1, 2, 3, or 4 μl of concentrated unknown, and bring volume up to 800 μl with water.

2) Add 200 μl concentrated Biorad reagent and incubate at room temperature for 5 minutes.

3) Assay absorbance at 595 nm.