GuidelinesfortheUseofAnalgesicsandTranquilizersinLaboratoryAnimal5
Anesthetic Induction and MaintenanceInjectable AnesthesiaAnesthetic induction using injectable anesthetics is fairly simple. It involves admininsistration of the drug and monitoring the depth of anesthesia. Supportive care may be needed. Maintenance of injectable anesthesia can be through repeated bolus doses of the drug or through a constant infusion. Infusion rates are calculated based on the clearance time of the ......閱讀全文
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal3
BarbituratesThe barbiturates are an acid ring molecule with various ring substitutes that imbue the drug with different properties. Barbiturates are a
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal7
ThermoregulationAnimals frequently become hypothermic during anesthesia because of inhalation of cold gases, exposure of body cavities to the room air
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal4
Other?AnalgesicsAnalgesics are pain relievers most often given after a surgery. Narcotic analgesics have already been described above. Nonsteroidal an
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal2
GeneralSome of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesia o
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal5
Anesthetic Induction and MaintenanceInjectable AnesthesiaAnesthetic induction using injectable anesthetics is fairly simple. It involves admininsistra
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal6
?Anesthetic?MachineThe best method of delivering an inhalant anesthetic is with an anesthetic machine. These machines precisely mix the gas with air o
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia??Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are?analgesia?(pain relief),?amn
Guidelines－for－Retroorbital－Bleeding－in－Laboratory－Rats－and－Mice
Materials Needed:Micro pipettes (l00 μl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samp
Yale－University－Policy－for－Aseptic－Amphibian－Survival－Surgery
Background:?Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the?Guid
Guidelines－for－Aseptic－Rodent－Survival－Surgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
siRNA－Design－Guidelines
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
Sea－Urchin－Animal－Maintenance
The following is written to help non-marine biologists use sea urchins occasionally in teaching; disregard if you have running sea water tanks in your
General－Laboratory－Methods－for－Tetrapyrroles
There are thousands of porphyrins and metalloporphyrins, and hundreds of new derivatives appear each year. This variety arises because the cyclic
Long－PCR－Reagents－and－Guidelines
George Church Lab, Harvard Medical SchoolPCR_protocol.html">http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.htmlEfficient Long PCR results fr
ELISA－Protocol－(General－Guidelines)
實驗概要Sandwich ?enzyme-linked immunosorbent assays (ELISAs) involve attachment of a ?capture antibody to a solid phase support. Samples containing known
ELISA－Protocol－(General－Guidelines)
實驗概要Sandwich ?enzyme-linked immunosorbent assays (ELISAs) involve attachment of a ?capture antibody to a solid phase support. Samples containing known
D熒光素鉀鹽/鈉鹽體內使用手冊（四）
表3：大鼠和小鼠使用不同的鎮靜劑藥物（IP=intraperitoneal; SC=subcutaneous; PO=per os(by mouth); IV=intravenous）藥物大鼠劑量（mg/kg）小鼠劑量（mg/kg）注解Tranquilizers/sedativesAcepromaz
LongPCR－Reagents－and－Guidelines
Long-PCR Reagents and Guidelinesfrom George Church as Modified from Cheng et al. (1)General Guidelines for Long-PCR Conditions and Enzyme Mixtures====
Experimental－Surgery2
Use of?Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized or wh
Thawing－－Incubating－Human－－Animal－Liver－Microsomes
實驗概要BackgroundThe ?liver is the major organ for metabolism of endogenous substrates as ?well as exogenous drugs. There are several in vitro tools avai
RNase－and－DEPC－Treatment:－Fact－or－Laboratory－Myth
Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not qu
RNA－Isolation－From－Animal－tissue－or－cell－culture
實驗概要This method is ?designed for most animal tissues and culture cells. For RNA isolation ?from fibrous tissue, follow the specialized protocol on pag
Apoptosis:－A－Laboratory－Manual－of－Experimental－Methods－Andrea－Cossarizza
THE CELL?1.?Morphological aspects of apoptosis?Walter Malorni, Stefano Fais & Carla Fiorentini?2.?Cell cycle?Miriam Capri & Daniela BarbieriTHE NUCLEU
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo
Cellular－－Molecular－Pathology－Branch
VisionTo provide scientific collaboration of excellence to National Toxicology Program (NTP)?(http://ntp.niehs.nih.gov/)?interdisciplinary research pr
ICBR－Flow－Cytometry－Core－Laboratory－Paraformaldehyde－Fixation－of－Cells
BackgroundThis fixation method is good for cells labelled by fluorochrome-conjugated antibodies to membrane antigens. It will stabilize the light scat
Laboratory－Investigation：新藥可預防致命核輻射
新研究發現對抗致命的輻射有了新突破。在核輻射暴露后24小時內，一種再生肽可以幫助顯著提高小鼠的生存時間。目前這項研究出版在自然出版集團的雜志《Laboratory Investigation》上。 德州大學醫學分部研究作者Carla Kantara是生物化學和分子生物學博士后，他說當小鼠處于致
Blood－collection－and－administration－of－fluids－and－drugs－(mouse)2
Intraperitoneal:Equipment:?Syringe and 23 to 27 g, 1/2 to 1-inch needle, preferably with a short bevel.Volume:?The volume injected IP into an adult mo
Preparation－of－Mitochondria－from－Rat－Liver
Preparation of Mitochondria from Rat LiverRat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawl
Magnetic－Depletion－of－SSEA4+－Undifferentiated－Embryonic－Stem－Cells
實驗概要Human ?embryonic stem cells can be differentiated into neural-, mesenchymal ?and hematopoetic stem cells. This product is intended for the magneti