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Description RNA extraction using TRI REAGENT. This method gives ample amout of RNA.Procedure It is 3 days procedure.Day 1:1. Harvest the cells and centrifuge at 2000 rpm for 5-10 minutes and pour off the media.2. Wash the cell pellet with PBS and add 10 ml TriReagent solution.3. Incubate at Room Temperature for 5 min and during incubation pass the cell suspension through Pipette.4. Collect the solutions in ......閱讀全文
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio
III. Methods for DNA isolationA. Large scale double-stranded DNA isolationThe method used for the isolation of large scale cosmid and plasmid DNA is a
My Experience Purifying RNA from E. coliRegarding RNA extraction, there is a horrible tendency of people to use kits for RNA extraction with bacteria.
實驗概要The PureLink? Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
This method was submitted by Jim Hutchins from the University of Mississippi Medical Center.The following is a great RT- PCR protocol develo
This method was submitted by Jim Hutchins from the University of Mississippi Medical Center.The following is a great RT-PCR protocol developed by
Most RNA isolation techniques currently in use have been developed for the processing of large quantities of material. These typically involve multipl
實驗概要The SuperScript? III One-Step RT-PCR System with Platinum? Taq High Fidelity is designed for sensitive, high-fidelity end-p
截止2020月7月27日，中國學者在Cell，Nature 及Science 發表了共計102項生命科學的研究成果，其中新冠肺炎領域占了近一半（共43篇）。iNature系統總結了這些研究成果： 按雜志來劃分：Cell 發表了30篇，Nature 發表了45篇，
IntroductionFluorescence in situ hybridization (FISH) has become a widely used method in genome and molecular genetic studies. The technique
IntroductionThe following protocol describes a procedure for the purification and cloning of miRNAs and other small RNAs in the 20-30 nucleotide
截至2019年12月23日，中國學者在Cell，Nature及Science在線發表了107篇文章（2019年的Cell ，Nature 及Science 已經全部更新），iNature團隊對于這些文章做了系統的總結： 按雜志來劃分：Cell 發表了31篇，Nature 發表了44篇，Scie
Comparison of normalisation methodsThere is an ongoing debate what is the best way to normalise qPCR data. Reference genes are the most common method,
Comparison of normalisation methodsThere is an ongoing debate what is the best way to normalise qPCR data. Reference genes are the most common me
實驗概要E.Z.N.A.? MicroElute? Total RNA Kit provides a rapid and easy method for the isolation of up to 50 ug of total RNA from small amount o
2019年上半年很快就結束了，iNature盤點了中國學者在Cell，Nature及Science發表的成果，我們發現總共有86篇（截至2019年6月24日），具體介紹如下： 4-6月發表的文章 【1】2019年6月21日，西北工業大學王文，中科院昆明動物研究所/BGI 張國捷及丹麥哥本哈根
Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not qu
A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves(See also
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation
Load your precipitated PCR samples into 2 consecutive lanes so as not to overload the lanes. For each different sample, I would run a separate ladder
AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are de
摘要：基因芯片技術是90年代中期以來快速發展起來的分子生物學高新技術，是各學科交叉綜合的嶄新科學。其原理是采用光導原位合成或顯微印刷等方法，將大量DNA探針片段有序地固化予支持物的表面，然后與已標記的生物樣品中DNA分子雜交，再對雜交信號進行檢測分析，就可得出該樣品的遺傳信息。基因芯片技術目前國內
主要內容如下：· RT-PCR· Competitive and Quantative
實驗步驟1. UV cross-linking of tissue culture cells 1) Remove media and add ice-cold PBS to cells (e.g. use cells grown in a 10 c
實驗概要The E.Z.N.A. Total RNA Kit can be modified for isolation of RNA from bacterial cultures. Only cells growing at log phase should be used. Measu
實驗概要The E.Z.N.A.? Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysi
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison
實驗概要Provides an easy and fast method for isolating total RNA from fibrous tissues which contains contractile proteins, connective tissue and colla
實驗概要This method is designed for most animal tissues and culture cells. For RNA isolation from fibrous tissue, follow the specialized proto
Preparing Lambda DNA1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required