FGFRINHIBITOR,SU5402,ONCRANIOFACIALDEVELOPMENTINCHICKENEMBRYOS
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epithelial-mesenchymal interactions by means of regulating cell proliferation and differentiation. FGF signaling is required for for limb bud and craniofacial development among other develpmental processes (Min et al. 1998; Ochuchi et al. 1997; Iseki et al. 1999; Crossley ......閱讀全文
2－Dimensional－Gel－Electrophoretic－Analysis－for－Chicken－Egg
Overview?? ? This protocol is a detail description of the procedure in performing 2D gel electrophoresis for illustrating the protein profile of the w
The－effect－of－temperature－on－the－embryonic－development－of－sea－urchins
Objective:?This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the external en
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Chorioallantoic－membrane－grafting－with－chick－embryo－limb－buds
ObjectiveThis experiment explores the ability of the chick chorioallantoic membrane (CAM) to support an excised limb bud from a donor embryo. The chic
The－effect－of－lithium－chloride－on－Lytechinus－variegates－embryos
PurposeThis experiment will study effects of lithium chloride on sea urchin development, forcusing on archenteron formation.IntroductionGastrulation i
Determining－the－effects－of－nicotine－on－bone－－in－chicks
ObjectiveThe purpose of this experiment is to determine how the development of bone and cartilage in a chick are affected by treating the embryo with
The－Developmental－Effects－of－Nicotine－on－Chick－Embryos
More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette
Dorsal－Lip－Transplant－in－Amphibian－Embryos
IntroductionIn early stages of development in amphibian embryos, very few cells' fates are rigidly determined. However, previous experiments show
Immunofluorescent－staining－of－Sea－Urchin－embryos
1. Transfer fixed embryos to microfuge tubes. Allow to settle for 10 minutes.Gently remove most of the liquid.2. Add 100 ul antibody to one tube and 1
The－Effects－of－NiCl2on－Spicule－Formation
The Effects of NiCl2on Spicule FormationJessica Ann Billet, Franklin and Marshall, Class of 2000Background and ObjectiveSea urchins exhibit radial hol
Zebrafish－whole－mou...
實驗概要Whole mount staining of Zebrafish embryos, now commonly used, requires extra steps to fix and permeabilize to ensure the egg membrane is perme
The－Effects－of－Ultraviolet－Light－on－the－Fertilization
Jill K. Flemming, Franklin & Marshall College, Class of 2001IntroductionThe objective of this project is to observe the effects of UV radiation on bot
多功能胚胎激光破膜儀（一）
Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis.T. Turetsky, E. Aizenman, Y. G
Ultraviolet－irradiation－impairs－epiboly－via－microtubules－in－Zebrafish
Introduction Zebrafish have transparentembryos that develop outside the mother. They develop rapidly, so that at 24 hours after fertilization, the em
多功能胚胎激光破膜儀（二）
A new approach to cyropreservation of large equine embryos by vitrification after blastocoel micromanipulation.J. Scherzer, R. A. Fayrer-Hosken, L. Ra
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mo
Zebrafish－Staging
In order to repeat a published experiment, or have someone else repeat yours, it is important to use the same materials. For developmental studies, th
Chick－or－Mouse－embr...
實驗概要The following procedure describes the procedure for whole mount staining of chick or mouse embryo’s. A similar procedure could be used for sta
Cell－death－detection－in－Xenopus－embryos－by－ELISA
Sample preparation Wash embryos 1 x in 25% MMR Remove excess buffer Add 10 volumes "incubation buffer", i.e. 50 μl for 5 embryos Lyse the embryos
Whole－mount－TUNEL－analysis－of－Xenopus－embryos
Fixation and pretreatmentDejelly albino embryos carefully in 2% Cystein (pH 7.8).Remove the vitellin membrane with two pairs of tweezers???????? (or c
Preparation－of－fixed－embryos－for－immunocytochemistry－and－AP－staining
1. Transfer 50 ml of embryo cultures to centrifuge tubes. Spin at 1500 rpm for 5 minutes. Check that you can see a pellet of embryos at the bottom.Qui
Shellless－cultures－of－chick－embryos
This experiment allows you to observe the development of a chick embryo outside its shell. Cultured chicks are also more accessible for manipulation.
Protocol－to－Count－Cell－Number－of－Preimplantation－Embryos
Protocol to Count Cell Number of?Preimplantation?Embryos?using Nuclear Staining with Hoechst 33342 or DAPI??Introduction?The following is a simple pro
Cytokine－induced－angiogenesis－in－chick－embryos.
Objective:The purpose of this experiment was to explore the effects of the growth factors bFGF and VEGF on blood vessel formation within the chorioall
Fate－Mapping－Axolotl－Embryos－in－Early－Gastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method us
Discovery－of－Elbasvir:－An－HCV－NS5A－Inhibitor－with－Broad－Genotype－Activity
分析測試百科網訊 2015年10月20 日，第十三屆國際新藥發明科技年會與第五屆藥物遞放系統研討會（SDDS-2015）于北京希爾頓逸林酒店同期舉辦并開幕。 中國默克公司執行董事 Peter T. Meinke 中國默克公司執行董事Peter T. Meinke帶來題目為《Disco
早期胚胎發育中的單胚胎細胞基因表達（二）
“We picked 42 genes to validate on the BioMark system,” Dr. Yao said. “We picked them to represent different functional categories.”“We used the F
Look－Ma,－No－Archenteron!－Sulfates－role－in－sea－urchin－early－development
Objective To observe the role sulfate plays in sea urchin gastrulation, and to replicate the findings of Karp and Solursh, that sea urchin embryos f
Chick－Embryo－staging
In order to repeat a published experiment, or have someone else repeat yours, it is important to use the same materials. For developmental studies, t