Table 2 Modulation of microRNA expression by IFNα—4 and 24 h after stimulation
| | Control | Interferon-alpha treated |
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4 h | 24 h | 4 h |
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| 24 h |
|---|
a | ME-15 | HuH7 | CHF | ME-15 | HuH7 | CHF | ME-15 | HuH7 | CHF | ME-15 | HuH7 | CHF |
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Validated microRNAs |
|
|
hsa-miR-19a | 13,617 | 6,726 | ?1.02 | 18,318 | 17,178 | ?0.07 | 19,226 | 17,409 | ?0.10 | 14,425 | 14,079 | ?0.02 |
|
|
hsa-miR-19b | 13,365 | 9,406 | ?0.42 | 25,463 | 22,438 | ?0.13 | 21,532 | 20,625 | ?0.04 | 18,039 | 17,440 | ?0.03 |
|
|
hsa-miR-30e-5p | 9,497 | 6,838 | ?0.39 | 13,045 | 11,321 | ?0.15 | 12,643 | 10,887 | ?0.16 | 13,230 | 11,809 | ?0.12 |
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|
hsa-let-7a | 15,244 | 4,335 | ?2.52. | 28,304 | 6,181 | ?3.58 *** | 22,226 | 6,386 | ?2.48*** | 30,449 | 5,612 | ?4.43*** |
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hsa-let-7b | 8,236 | 539 | ?14.28*** | 12,563 | 475 | ?25.44*** | 12,150 | 500 | ?23.30 *** | 12,309 | 452 | ?26.24*** |
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hsa-miR-203 | 907 | 325 | ?1.79** | 1,155 | 348 | 2.32*** | 1,302 | 359 | ?2.63*** | 1,186 | 5,757 | ?1.07** |
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hsa-miR-130b | 5,700 | 8,316 | ?0.46 | 13,023 | 13,880 | 0.07 | 9,994 | 14,055 | 0.41 | 12,053 | 12,432 | 0.03 |
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hsa-miR-455 | 2,677 | 2,604 | ?0.03 | 3,944 | 5,285 | 0.34* | 3,437 | 5,927 | 0.72** | 3,262 | 5,342 | 0.64* |
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b | ME-15 | HuH7 |
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4 h | 24 h | 4 h |
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| 24 h |
?IFNa | +IFNa | CHF | ?IFNa | +IFNa | CHF | ?IFNa | +IFNa | CHF | ?IFNa | +IFNa | CHF |
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Interferon-regulated microRNAs |
|
|
hsa-miR-33b | 1,226 | 3,484 | 1.84** | 2,888 | 2,318 | ?0.25 | 476 | 1,113 | 1.34** | 1,306 | 1,851 | 0.42*** |
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hsa-miR-33 | 2,631 | 7,352 | 1.79 | 9,774 | 5,764 | ?0.70* | 1,887 | 6,645 |
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