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  • 發布時間:2019-05-19 16:31 原文鏈接: RNA點線雜交

    RNA點線雜交(Dot and Slot blotting

    ·         RNA dot blot and slot blot (Beverly Faulkner-Jones)
    This methods allows the rapid analysis of numerous small samples for the sequence of interest and is less time consuming than the gel electrophoresis methods. 'Dots' or 'slots' of RNA are made onto a filter using a manifold and the filter is then hybridized with a labeled probe. Partially degraded RNA can also be used with good semi-quantitative results. However, high false positive rates need careful use of appropriate controls


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