Abstract: 基于安捷倫 1200高分離快速液相色譜(RRLC)/組合離子源的 6430A三重串聯四極桿液質聯用系統開發了血清中
25-OH D2 和 25-OH D3 的快速定量分析法。 固相萃取最大限度降低了基質干擾和離子抑制，同時還能自動化運行。在 5至 100 ng
/ mL的濃度范圍內具有優異的定量線性度響應(R2 > 0.99)，而且分析時間只有五分鐘，日間精密度遠遠低于
10%。血清中的檢測限(LOD)和定量限(LOQ)分別是 2 ng/mL 和 3 ng/mL。 A rapid method for the
quantification of 25-OH D2 and 25-OH D3 in serum was developed using the
Agilent 1200 Series Rapid Resolution LC (RRLC) system coupled with an
Agilent 6430 Triple Quadrupole LC/MS with multimode ionization (MMI).
Solid phase extraction minimizes matrix interferences and ion
suppression, while enabling automation. Excellent linearity of
quantification (R2 > 0.99) was obtained in the range of 5 to 100
ng/mL, with run times of only 5 min, and day-to-day precision was well
below 10%. The limits of detection (LOD) and limits of quantification
(LOQ) were 2 ng/mL and 3 ng/mL respectively, in serum.
Keywords: 1200，快速高分離液相色譜，多模式進樣口，血液，癌癥，鈣，磷 1200, RRLC, 6430, MMI, blood, cancer, calcium, phosphorus
Publication Number: 5990-4549CHCN
Last Updated: 9/3/2010
Number of Pages: 4
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