WIKI資訊
One defense against viral infection is provided by PKR, double-stranded RNA activated protein kinase. When PKR interacts with dsRNA found in cells during viral infection, PKR phosphorylates itself and cellular proteins including the translation factor elF2a and the transcription factor NF-kB. The repression of translation caused by phosphorylation of elF2a prevents cells from producing viral proteins and creating inf......閱讀全文
2.3 倍增階段 siRNA在RNA依賴性RNA聚合酶(RdRP)的作用下，以mRNA為模板，siRNA為引物，擴增產生足夠數量的dsRNA作為底物提供給Dicer酶，產生更多的siRNA， 可再次形成RISC，并繼續降解mRNA，從而產生級聯放大效應
早期的 RNAi 技術可用在研究與胚胎發育相關基因的功能上，但是由于細胞分裂造成 dsRNA 的稀釋，使得這種方法在研究成體的基因功能時有一定的局限性。為彌補早期 RNAi 技術的不足，Tavernarakis 等將 RNAi 技術做了一些改進及更動，將目的基因之標的序列以反向重復的方式，由
Figure 2. Silencer ? siRNA Validation Data Generated Using Applied Biosystems TaqMan? Gene Expression Assays. The indicated Silencer Validated siRNAs
cDNA LibrariesIsolation of corresponding genetic informationInstead of synthesizing a desired gene, can we used the amino acid information to directly
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
Ambion and Applied Biosystems have joined forces to provide a complete convenient, solution for performing gene silencing experiments and validating t
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to h
MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2aMicroRNAs are positive and negative r
RNAi target selection rules:Targeted regions on the cDNA sequence of a targeted gene should be located 50-100 nt downstream of the start codon (ATG).S
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by we
Argonaute - A family of proteins containing multiple domains and involved in RNA interference (RNAi). Argonatue is the main component of RNAi eff
RNA interference (RNAi) is a phenomenon in which the introduction of double-stranded RNA (dsRNA) into a diverse range of organisms and cell types caus
1. Introduction and BackgroundThere is a great need for general methods to characterize the proteins that contemporary biology makes available. The li
RNA干擾(RNA interference ,RNAi) 現象是一種進化上保守的抵御轉基因或外來病毒侵犯的防御機制。將與靶基因的轉錄產物mRNA 存在同源互補序列的雙鏈RNA(double strand RNA ,dsRNA) 導入細胞后,能特異性地降解該mRNA ,從而產生相應的功能表型缺失
Assay of superoxide dismutase activity by combining electrophoresis and densitometryAbstract. A modified technique was developed to assay superoxide d
Terminal differentiation of cells is often accompanied by repression of cellular proliferation, suggesting that there is a mechanism by which these ce
截至2019年12月23日，中國學者在Cell，Nature及Science在線發表了107篇文章（2019年的Cell ，Nature 及Science 已經全部更新），iNature團隊對于這些文章做了系統的總結： 按雜志來劃分：Cell 發表了31篇，Nature 發表了44篇，Scie
Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting mess
結論· 我們在C1TM單細胞自動制備系統開發了一種簡潔的實驗方案，能以最少的手工操作，在不到24小時內，平行處理高達96個單細胞，對其miRNA表達譜進行分析。· C1 miRNA STA實驗方案使用了Life Technologies為miRNA優化過的試劑。特別的，Meg
FL3500雙調制葉綠素熒光儀 （新升級型號為FL6000） FL3500雙調制葉綠素熒光儀是專門用于對藍綠藻或綠藻等微藻，葉綠體或類囊體懸浮物，乃至葉片進行光合作用研究的強大科研工具。儀器具備雙通道測量控制，可控制測量樣品的溫度，并配備單翻轉光（STF），內置多種可用戶自行修改的測量程序
來自北京大學生命科學學院、美國國立人類基因組研究所和加州大學生物系的研究人員，最近在利用逆轉錄病毒插入法引發斑馬魚全基因組范圍內基因突變的研究中取得重大進展。文章刊登于7月18日在線版《PNAS》。 研究人員采用其研制的一組技術，用假性逆轉錄病毒（pseudotyped retroviruses）
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
生命極其脆弱，我們每天在電子輻射、紫外線、霧霾等等各種外部環境及細胞代謝產物等內源因素影響下，我們生命的核心-DNA都會受到不同程度的損傷，其中DNA雙鏈斷裂（DSBs，Double strand breaks）是損傷中最為嚴重的一種，然而生命卻又極其強大，我們無時無刻不在受傷，也無時無刻不
【干貨】拯救你受傷的DNA-NHEJ與HR生命極其脆弱，我們每天在電子輻射、紫外線、霧霾等等各種外部環境及細胞代謝產物等內源因素影響下，我們生命的核心-DNA都會受到不同程度的損傷，其中DNA雙鏈斷裂（DSBs，Double strand breaks）是損傷中最為嚴重的一種，然而生命卻又極
5月14日的《Nature》雜志以封面文章形式發表了德克薩斯大學西南醫學中心劉一的一篇RNAi研究前沿文章，劉一教授發現了一類新的RNAi分子，qiRNA。人們對RNAi的了解又深入了一步。 為了讓讀者更進一步的了解qiRNA，生物通記者采訪了文章的通訊作者劉一教授，百忙中的劉一教授爽
RNAi 技術的機理與應用 關于 RNAi 技術 RNA 干擾（RNA interference,RNAi）是指在進化過程中高度保守的、由雙鏈 RNA( double-stranded RNA，dsRNA) 誘發的、同源 mRNA 高效特異性降解的現象。 RNAi 受到追捧的
RNAi是Napoli CD等在試圖向紫色矮牽牛花轉導色素合成基因，用以增加其花色時發現的。結果出乎預料，轉基因的植株不僅沒有新基因的表達，反而自身的色素合成也減弱了，一些轉基因的花出現了全白色或部分白色。他們把這種導入的基因未表達和植物本身合成色素基因的失活現象命名為共抑制(cosupp