WIKI資訊
Packaging of DNA into chromatin allows the cell to store its genetic information efficiently and has an important role in regulating gene expression. Recent studies have revealed that chromatin structure can be altered by covalent modification of nucleosomes, in particular acetylation and methylation of the core histone tails. Histone hyperacetylation usually characterizes active chromatin, while histone deacetylatio......閱讀全文
The siRNA user guide (revised May 6, 2004) Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysa
Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed t
隨著人類基因組計劃（human genome project ）在2003年順利完成，基因組測序技術取得了長足的進步，這直接導致了每兆基因組成本的大幅下降以及檢測的基因組數量越來越多。人們對基因組的復雜性深感震驚，這也引導著測序技術的進一步發展。最近的一些突破性技術使得測序技術在更短的時間內可以
應用WGS正在成為NGS中最廣泛的應用。通過該技術并且結合生物學應用，研究人員可以獲得基因組信息中最值得注意的信息73。舉例來說，2012年，Ellis等報道了基因與乳腺癌患者芳香酶抑制劑（aromatase inhibitor）治療法之間的關聯。他們指出突變，后果與診斷之間的關聯，同樣還有
Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome a
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
2019年4月8日，中國科學院上海植物逆境生物學研究中心何躍輝課題組和杜嘉木組合作在Nature Plants發表題為“Embryonic resetting of the parental vernalizedstate by two B3 domain transcription facto
Argonaute - A family of proteins containing multiple domains and involved in RNA interference (RNAi). Argonatue is the main component of RNAi eff
截至2019年12月23日，中國學者在Cell，Nature及Science在線發表了107篇文章（2019年的Cell ，Nature 及Science 已經全部更新），iNature團隊對于這些文章做了系統的總結： 按雜志來劃分：Cell 發表了31篇，Nature 發表了44篇，Scie
實驗概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
RNA interference (RNAi) is a phenomenon in which the introduction of double-stranded RNA (dsRNA) into a diverse range of organisms and cell types caus
美國當地時間2月8日，美國工程院公布2017年入選院士名單，共新增84位院士及22位外籍院士。其中有多位著名華人學者，包括黃永剛、沈向洋、張東曉、叢京生、陳向力等人。 美國國家工程院院士是工程界最高榮譽之一，授予那些在工程領域內從事研究、實踐和教育并作出卓越貢獻的人士；以及在新興和發展中的技
TROUBLESHOOTINGNo products are visible in any lane.1. Potential Problem: PCR amplification is not initiated.Recommendations:a. Confirm that all PCR co
2019年上半年很快就結束了，iNature盤點了中國學者在Cell，Nature及Science發表的成果，我們發現總共有86篇（截至2019年6月24日），具體介紹如下： 4-6月發表的文章 【1】2019年6月21日，西北工業大學王文，中科院昆明動物研究所/BGI 張國捷及丹麥哥本哈根
實驗概要This method is use to extract short RNAs from plant tissue. Some of the variables (e.g. centrifugation speeds×, precipitation
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
利用Mastercycler X50系列PCR獨有的2D梯度實現高效的PCR條件優化Ultimate PCR Optimization with Eppendorf Mastercycler? X50 2D-gradientArora Phang, Tim Schommartz,Eppe
RNAi GlossaryDicer - Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNAs. Dicer processes long d
Figure 2. Silencer ? siRNA Validation Data Generated Using Applied Biosystems TaqMan? Gene Expression Assays. The indicated Silencer Validated siRNAs
Tumorigenesis is known to be a multistep process in which defects in various cancer genes accumulate. Epigenetic modifications, most importantly DNA m
在免疫學實驗中，比如WB，IHC，IF等，大家都知道選著好正確的一抗，往往實驗就成功了一大半，因此，往往很多研究者便會忽視對二抗，以及其它試劑盒品質的追求。二抗是一種相對而言比較穩定的產品，包裝大，因此很多實驗室都會公用一個二抗。但如果二抗沒有選擇好的話，影響的可能就是一整個實驗室或者課題組了。艾美
The vitamin D receptor, VDR is the mediator of all genomic actions of vitamin D3 and its analogs. It belongs to a family of ligand induced transcripti
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio
The most recognized mechanism by which peroxisome proliferators regulated gene expresssion is through a PPAR/RXR heterodimeric complex binding to a pe
Hypoxia (or low O2 levels) affects various pathologies. First, tissue ischemia, a variation in O2 tension caused by hypoxia/reoxygenation, can lead to
The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive
Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to h
經過特殊的算法，我們得到了2018年前10個月中國生物醫學風云榜人物及最火爆的3個重大學術界事件，能夠上榜的風云人物/事件，都曾長時間占據過100多個公生物醫學公眾號的頭版頭條。 在此，我們精選了其中的3個事件及16位風云榜人物。我們對其進行了劃分，分別是：6星級的3個事件，分別位諾貝爾獎，國
Drug addiction is associated with long-term behavioral changes, suggesting a long-lived transcriptional regulator that responds to chronic drug exposu
Liver is the major site for carbohydrate metabolism (glycolysis and glycogen synthesis) and triglyceride synthesis (lipogenesis). While insulin was lo