WIKI資訊
實驗概要The E.Z.N.A.? family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is Omega Bio-Tek, Inc.’s (OBI) new HiBind? matrix that specifically, but reversibly, binds DNA or RNA under certain optimal conditions, while allowing proteins and other contaminants to be removed. ......閱讀全文
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
實驗概要This Protocol is designed to isolate 500-1200 ug of high Copy-Number plasmids or 50-400 ug of low Copy-Number Plasmids from 200 ml overnight c
實驗概要The E.Z.N.A.? family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a
實驗概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind? technology with the time-tested consistency of alkaline-SDS lysis of
實驗概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind? technology with the time-tested consistency of alkaline-SDS lysis of
實驗概要DNA isolation from fish or avian blood sample can be difficult because it contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA
實驗概要The E.Z.N.A.? Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysi
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
實驗概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
實驗概要This Protocol is designed to isolate 500-1200 ug of high Copy-Number plasmids or 50-400 ug of low Copy-Number Plasmids from 200 ml overnight c
實驗概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
實驗概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
實驗概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
實驗概要The E.Z.N.A.? family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids fr
Preparing Lawn Cells for M13 Cloning (Life Technologies)Lawn cells require the F' episome for M13 infection and may be prepared St
DNA電泳（主要內容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA fro
III. Methods for DNA isolationA. Large scale double-stranded DNA isolationThe method used for the isolation of large scale cosmid and plasmid DNA is a
E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo
Digestion of Proteins and Extraction of Peptides from an Acrylamide GelSherry Niessen, Ian McLeod and John R. Yates IIIDepartment of Cell Biology, The
DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Imple
實驗概要試劑盒，操作簡單，稍有點貴，還可以接受。實驗材料blood實驗步驟Protocol: DNA Purification from Blood or Body Fluids(Spin Protocol)This protocol is for purification of total (ge
IntroductionThe efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replica
實驗概要The efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA &nb
· Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in
As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of
C. elegans RNA Isolation and RT-PCRReagents Needed:M9 (common stock)Trizol (stored at 4oC)chloroform2-propanol70% EtOHRNase-free H2OiScript cDNA Synth
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
MaterialsFor purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results.Do not autoclav
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
C. Restriction digestionRestriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restrict