Basicproceduresforbacteriaculture1
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated phenol is added to an aqueous DNA sample in a microcentrifuge tube. The mixture is vigorously vortexed, and then centrifuged to enact phase separation. The upper, aqueous layer carefully is removed to a new tube, avoiding the phenol interface and then is subjected to t......閱讀全文
Basic－procedures－for－bacteria－culture2
E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo
Basic－procedures－for－bacteria－culture1
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
Bacteria－Growth－and－Culture－Bacteria－Growth－and－Culture
Flagella and motilitymonotrichous?flagella - the bacterial cell has a single flagellaperitrichous?flagella - the bacterial cell has several flagella w
Bacteria－Culture－Protocol
Bacteria Culture ProtocolBy 徐曉政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce
細菌培養
Preparing Overnight Bacteria Culture?(LaboratoryExperiments.com)This is a basic procedure for high school students and useful for those who are new to
Preparing－Overnight－Bacterial－Culture
Materials: Sterile LB medium (Luria-Bertani Medium) with or without antibiotic: water 500 ml bacto-tryptone 10 g bacto-yeast extracts 5
Preparation－of－Bacterial－Proteins－for－Analysis－by－2DPAGE
The following protocol has been developed for preparing soluble bacterial proteins in a form suitable for analysis by 2D-PAGE. The procedure was prin
Sterile－Technique
Good sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techn
Aseptic－Technique－and－Good－Cell－Culture－Practice
AimTo ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross con
無菌化技術
Sterile TechniqueGood sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein
Aseptic－Techniques
Aseptic techniques ensure that all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi, mycoplas
Protein－Staining－Procedures
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Basic－PCR
實驗概要The ?following basic protocol serves as a general guideline and a starting ?point for any PCR amplification. Optimal reaction conditions (incubati
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures?(Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures?(Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
Embryonic－limb－bud－culture－in－media
Early in embryonic development, the region of the chick embryo which is determined to form a limb first differentiates from the rest of the embryo1. T
Differential－cDNA－Screening－Procedures
Differential cDNA Screening ProceduresThe protocols listed refer to cDNA library construction and preliminary differential screening procedures. They
FACS－Procedures－for－Apoptosis－Detection
Materials:Hoechst?33258?(Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500μg/ml (50μl stock + 950μl PBS).7-Amino-actinomycin (Sigma A-94
Testing－for－Bacteria－and－Fungi
AimIn cases of gross contamination the naked eye may identify the presence of bacteria and fungi. However, it is necessary to detect low-level infecti
Biosynthesis－of－Proline－in－Bacteria
Among the twenty amino acids encoded by the genetic code, proline is unique in having a cyclic structure with its side chain connected to the amino gr
Basic－Mechanisms－of－SUMOylation
Like ubiquitin, SUMO (small ubiquitin-related modifier) proteins are small protein tags that are conjugated to proteins to modify their function. The
Basic－ELISA－Protocol
實驗概要? ? ? ? There are many different types of ELISAs, which can detect the presence of protein in serum or supernatent. One of the most common typ
質粒的小量制備
·?????????Standard (alkaline lysis) Mini-Prep?(Goldberg Lab)Standard protocol for mini-prep and recipe for solution I, II and III.Alkaline Lysis Minip
質粒的小量制備
·?????????Standard (alkaline lysis) Mini-Prep?(Goldberg Lab)Standard protocol for mini-prep and recipe for solution I, II and III.Alkaline Lysis Minip
Culturing－BG01V－Human－Embryonic－Stem－Cells－with－Mouse－Embryonic－Fibroblast
If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Med
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1.?Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2.?All women reported
Streaking－Bacteria－for－Single－Colonies
1. Initial inoculum:- From solid media, touch an isolated colonies with a sterile applicator or toothpick.- From liquid media, touch the culture with
Biosynthesis－of－Tryptophan－in－Bacteria－and－Plants
The aromatic amino acid tryptophan is an essential nutrient, meaning that humans and animals do not themselves have the biosynthetic machinery to synt
Biosynthesis－of－Chorismate－in－Bacteria－and－Plants
The biosynthesis of all three aromatic amino acids (tryptophan, tyrosine and phenylalanine) begins with the metabolic intermediate chorismate. The bio