TroubleshootingforPCRandmultiplexPCR
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles.COMPONENTVOLUMEFINALCONCENTRATION1.autoclaved ultra-filtered water (pH 7.0)20.7μL-2.10x PCR Buffer*2.5μL1x3.dNTPs mix (25 mM each nucleotide)0.2μL200 μM (each nucleotide)4.primer mix (25 pmoles/μL each primer)0.4μL0.4 μM (each primer)5.Taq DNA polymerase (native enzyme)0.2μL1 Unit/25 μL6.genomi......閱讀全文
Troubleshooting for PCR and multiplex PCR
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles.COMPONENTVOLUMEFINALCONCE
多重PCR（Multiplex PCR）
一般PCR僅應用一對引物，通過PCR擴增產生一個核酸片段，主要用于單一致病因子等的鑒定.多重PCR(multiplex PCR)，又稱多重引物PCR或復合PCR，它是在同一PCR反應體系里加上二對以上引物，同時擴增出多個核酸片段的PCR反應，其反應原理，反應試劑和操作過程與一般PCR相同.??? 多
多重PCR（Multiplex PCR）
一般PCR僅應用一對引物，通過PCR擴增產生一個核酸片段，主要用于單一致病因子等的鑒定.多重PCR(multiplex PCR)，又稱多重引物PCR或復合PCR，它是在同一PCR反應體系里加上二對以上引物，同時擴增出多個核酸片段的PCR反應，其反應原理，反應試劑和操作過程與一般PCR相同.??? 多
Authentication of Medicinal Plants by SNP-Based Multiplex PCR
Highly variable intergenic spacer and intron regions from nuclear and cytoplasmic DNA have been used for species identification. Noncoding internal
Multiplex PCR Method to Discriminate Artemisia iwayomogi from Other ...
Some plants in the genus Artemisia have been used for medicinal purposes. Among them, Artemisia iwayomogi , commonly referred to as “Haninjin,” is
來自耶魯大學的PCR常見問題的精辟總結
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles.?COMPONENTVOLUMEFINAL CON
PCR實驗指導與常見問題分析-1
CONTENTPCR guide: a discussion of the main parameters influencing the outcome of the PCR and multiplex PCR reaction in 16 pages/sections and using ove
PCR實驗指導與常見問題分析-6
Non-denaturing PAA gelsTo separate PCR products differing in only a few bp in length (for example, microsatellite markers), 6-10% PAA gels need to be
PCR實驗指導與常見問題分析-4
Fig. 25.?Multiplex PCR of mixtures A-D comparing PCR programs with 2 (green) and 1 (yellow) minute extension time at 54° C annealing temperature. Comp
PCR實驗指導與常見問題分析-7
11. All products in my multiplex reaction are weak. How can I improve the yield? Decrease annealing time in small steps (2o C) Decrease ex