PlatinginTopAgar
1. Warm plates to room temperature before use. Cold plates causes the top agar to solidify irregularly. DO not warm plates to 37° as the top agar will take forever to solidify.2. Prepare top agar as the appropriate liquid medium with 0.7 agar. Keeping 100 mL bottles is convenient. For phages, use λ top agar, which is less rich and yields bigger plaques.3. Melt top agar in the microwave completely. Allow the agar to b......閱讀全文
Plating in Top Agar
1. Warm plates to room temperature before use. Cold plates causes the top agar to solidify irregularly. DO not warm plates to 37° as the top agar will
SOFT AGAR ASSAY FOR COLONY FORMATION
Note: All volumes are calculated to cater for four plates per point. Base Agar 1. Melt 1% Agar (DNA grade) in microwave, cool to 40 in a waterbath
Easy Way to Clone Genes From a Phage Library
Easy Way to Clone? The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97. The overall sequence of ev
Phage Titer
IntroductionLambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clon
Cell counting/plating
OverviewCount the number of viable cells in a culture via dilution plating. The following assumes E. coli cells but it should apply to any type of cel
Pouring Plates
1. For rich media, weigh out appropriate ingredients and place into a flask. Add water until appropriate volume. Use a flask at least 2 times larger
Thawing and Plating Cryopreserved Hepatocytes
實驗概要This ?protocol covers thawing and prep of cryopreserved hepatocytes for ?applications such as metabolic stability, intrinsic clearance, enzyme ?in
Bacterial cell culture
Materials Glass culture tubes with metal caps and labels Growth medium, from media room or customized Glass pipette tubes Parafilm Equ
Preparation of Agar plates
Prepare media and add 1.5 agar before autoclaving it (15g per liter).? After autoclavation, cool the media in a 55 degree waterbath. Do not allow? th
Noble Agar Assay
DescriptionCancer cells do not show anchorage and contact inhibition of growth. To assess the anchorage and contact independent growth of cells, noble