FACSProceduresforApoptosisDetection
Materials:Hoechst 33258 (Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500μg/ml (50μl stock + 950μl PBS).7-Amino-actinomycin (Sigma A-9400)stock: 200μg/ml in dH20 (40)12x75mm snap cap tubes (Falcon 2054).Controls Required:Cells only (no treatment/no stain)Treated cells with Hoechst onlyTreated cells with 7-AAD onlyUntreated cells with both stainsProcedure:Resuspend at least 0.5 x 106 cell......閱讀全文
FACS Procedures for Apoptosis Detection
Materials:Hoechst?33258?(Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500μg/ml (50μl stock + 950μl PBS).7-Amino-actinomycin (Sigma A-94
Detection Of Cell Viability And/Or Apoptosis By Flow Cytometry (FACS)
Viable?cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells in ear
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
ASENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
Protein Staining Procedures
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Coenzyme A Detection
實驗概要The experiment provided ?an ?easy, convenient assay to measure the CoA level in a variety of ?biological samples. In the assay, free CoA is specif
Cell Sorting By FACS
Currently, the Moflo instrument (Dakocytomation) is used to sort cells at the AECOM FACS facility.Sorting is performed by the person in charge at the
Apoptosis: Miniassay
1) Aliquot 5 X 106 total cells in 5-8 ml 2% media. 2) Add 30 μl 3H-thymidine. 3) Incubate for approximately 16 hours under normal growth conditions
Apoptosis Induction
IntroductionWhen studying induction of apoptosis via a cell surface molecule, it is important to first ascertain surface expression of the molecule of
Differential cDNA Screening Procedures
Differential cDNA Screening ProceduresThe protocols listed refer to cDNA library construction and preliminary differential screening procedures. They