FluorescenceInSituHybridizationusingTSA?
實驗概要This protocol describes steps for fluorescent in situ hybridization (FISH) to Drosophila embryos using Tyramide Signal Amplification (TSA?), and was developed in collaboration with Ethan Bier and Dave Kosman at UCSD. This protocol is demonstrated with Drosophila embryos, but should be easily amenable to any specimen or tissue. TSA? provides enzymatic fluorescent signal amplific......閱讀全文
Fluorescence Mounting Medium (Antifade)
Materials Needed20ml glass scintillation vialSmall stir barFoilGlycerol1X PBSPipets* P-phenylenediamine ( EMD Chemicals Inc. Cat# PX0730)Carbonate-Bic
Fluorescence In Situ Hybridization using TSA?
實驗概要This ?protocol describes steps for fluorescent in situ hybridization (FISH) ?to Drosophila embryos using Tyramide Signal Amplification (TSA?), and
Immunohistochemisty-Fluorescence Protocol-2
Suitable for use on single-cell suspensions from peripheral blood, lymphoid tissue or cultured cell-lines.Sample Preparation and FixationHarvest cells
Immunohistochemisty-Fluorescence Protocol-1
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'&
熒光分析法（fluorescence analysis
熒光光譜基礎； 蛋白質的熒光特性； 熒光分光光度計的結構和原理。吸收光譜和熒光光譜能級躍遷示意圖 （一）熒光的產生 某些物質受紫外光或可見光照射激發后能發射出比激發光波長較長的熒光。此化學物質能從外界吸收并儲存能量（如光能、化學能等）而進入激發態，當其從激發態再回復到基態時，過剩的能量可以電磁輻射的
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing
Fluorescence Procedures forthe Actin andTubulin Cytoskeleton in Fixed Cells
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed CellsActin: Louise CramerTubulin: Arshad DesaiGeneral StrategyWe typically wor
均相熒光免疫測定（homogeneous fluorescence immunoassay）
均相熒光免疫測定（homogeneous fluorescence immunoassay）是根據1972年Rubenstein等建立的均相酶免疫測定法（HEI）發展形成的一種新型免疫熒光分析技術。所謂“均相”是指在反應結束后無須對游離和結合的標記物進行分離，直接測定即可。均相熒光免疫測定是利用
熒光原位雜交（Fluorescence in situ hybridization,FISH）
實驗原理熒光原位雜交（Fluorescence in situ hybridization FISH）是一門新興的分子細胞遺傳學技術，是20世紀80年代末期在原有的放射性原位雜交技術的基礎上發展起來的一種非放射性原位雜交技術。目前這項技術已經廣泛應用于動植物基因組結構研究、染色體精細結構變異分析、病
Fluorescence Procedures forthe ActinandTubulin Cytoskeleton inFixed Cells-2
Actin Cytoskeleton Methanol fixation Fix in -20oC methanol for 1-2.5 minutes Rinse in TBS Permeabilize in TBS-0.5% TX for 10 minutes Rin