AutomatedExtractionNormalizedDNABuccalKit
實驗概要This section provides a general protocol for automated isolation of genomic DNA from human buccal cell swabs in a 96-well format using the ChargeSwitch?gDNA Normalized Buccal Cell Kit (Catalog no. 11020-10). Use this general protocol to develop the script for your liquid handling robot.主要試劑1. ChargeSwitch? Lysis Buffer (L11)2. Proteinase K 3. ChargeSwitch? Magnetic Beads4. ChargeSwitch? P......閱讀全文
DNA isolation extraction
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolati
Streamlined DNA Extraction Protocol
This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure wi
Fungal Genomic DNA Extraction
Overview High throughput of many fungal isolates can be achieved by growing axenic cultures in either (a) 1.5mL microfuge tubes, half full with liqu
DNA EXTRACTION PROCEDURE - GENERAL
Grow cells overnight in 500 ml broth medium. Pellet cells by centrifugation, and resuspend in 5 ml 50 mM Tris (pH 8.0), 50 mM EDTA. Freeze ce
Fungal Genomic DNA Extraction
Overview High throughput of many fungal isolates can be achieved by growing axenic cultures in either (a) 1.5mL microfuge tubes, half full with liqu
Fungal Genomic DNA Extraction
實驗概要 This procedure does not require phenol extraction. The DNA is pure enough for restriction digests, PCR and genomic library construction. Hi
Automated Genomic DNA Extraction
實驗概要This section ?provides a general protocol for automated isolation of genomic DNA from ?10-20 μl blood samples in a 96-well format using the Charge
Genomic DNA Extraction - PureLink?
實驗概要The ?PureLink? Genomic DNA Purification Kit allows rapid and efficient ?purification of genomic DNA. The kit is designed to efficiently isolate ?g
DNA Extraction from Blood
實驗概要The ChargeSwitch? ?gDNA Purification Kits allow rapid and efficient purification of ?genomic DNA from small volumes of human blood. After preparin
DNA Extraction from Tissue
實驗概要DNA extraction from tissue.主要試劑Extraction buffer100 mM Tris-HCl (pH 8.0)?????100 mM EDTA (pH 8.0)?100 mM Na-Phosphate (pH 8.0)???1.5 M NaCl1% CTAB