XenofreeCultureofNeuralStemCells
實驗概要Neural stem cells (NSCs) derived from human embryonic stem cells (hESCs) have the potential to help provide understanding for human neurogenesis and for potential cell therapy applications to treat Parkinson’s Disease or spinal cord injuries. Standard methods of culturing NSCs raise concerns about pathogen cross-transfer from nonhuman sources or contamination with non-neural ce......閱讀全文
Culture of BEND Cells (Bovine Endometrial Cells)
Culture of BEND Cells (Bovine Endometrial Cells) Charles E. Krininger, III and Peter J. Hansen? Dept. of Animal Sciences, University of Florida Thi
Differentiate ES cells into glial cells and neurons
Day -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells. ___________________ Day 1:?Tryp
Differentiating Neural Stem Cells into Neurons and Glial Cells
實驗概要The protocols in ?this section describe the steps involved in differentiating neural stem ?cells (NSC) to neurons, astrocytes, and oligodendrocyte
Growing cells
No two cell lines behave exactly the same, so you must learn the peculiarities, or personality, of each of the cell lines with which you work. Irresp
Trypsinizing cells
There are many procedures with which to trypsinize cells. All include washing the cell monolayer with TD, or in rare cases, with VE. This removes ser
Lyophilizing Cells
Inoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized. Incubate the culture at 37°C with vig
Lyophilizing Cells
Lyophilizing Cells Inoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized. Incubate the c
Freezing Cells
1) Keep prepared solutions on ice.2) Determine total cell count of cells to be frozen. (e.g. 1 X 108 )3) Determine number of vials to be frozen. (e.g.
Isolation of papillary cells
Isolation of renal papillary cells1.?For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
Electroporation of ES cells
Cells are routinely passaged two days prior to electroporating. Usually one 10 cm plate at approximately 80% confluency will provide enough cells for