AMethodforMicroScaleIsolationandPurificationofGangliosides
A Method for Micro-Scale Isolation and Purification of GangliosidesStephan Ladisch~Director, Center for Cancer and Transplant Biology, Children's National Medical Center, Washington, D.C. 22010 This solvent partition method permits the isolation of gangliosides from small samples and from samples in which the ganglioside concentration is low, especially relative to the concentration of potentially contaminat......閱讀全文
Protein－A－Purification－of－Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buff
Protein－purification;－actin
Protein purification; actin ? ? ?Overview?? ACTINThe most abundant muscle and non-muscle cytoskeletal protein. MW 42 kDa, 374/375 amino acids; various
DNA電泳
DNA電泳（主要內容如下）??Preparation of Agarose Gel and Electrophoresis??Extraction of DNA From Agarose Gel??Extraction of DNA from Acrylamide Gels??DNA Marker?
Method:－Lymphocyte－Transformation
Method: Lymphocyte TransformationMay 30, 1990Rosalie VeilePrinciple:Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocyte
其它PCR方法
·?????????Standard PCR Protocol?(Molecular Biology Techniques Manual)The followings are described in detailRecommended Reagent ConcentrationsRecommend
Column－Purification－of－Demethylated－Sphingomyelin
Packing column:1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of chloroform.2) Place a small portion of glass wool at the base of the colu
Purification－of－GST－Fused－Proteins
Day 1Set up an overnight culture in 100 ml LMM broth or 100 ml terrific broth containing 100ul 100 mg/mlAmpDay 2Add 40-50 ml o/n culture to 1 lt terri
Protein－Expression－and－Purification－Protocol
Step 1:?Transform?appropriate DNA plasmid into BL21(DE3)?E. coli?cells. These cells must be competent. (Protocol for how to make competent cells.)a) T
Protein－G－Purification－of－Antibodies
1. Reagent and Materials(1) Hi-Trap Protein G Column (Pharmacia Biotech #17-0404-01)(2) 20 mM Sodium Phosphate Buffer, pH 7.01.084 g NaH2PO4, anhydrou
Isolation－of－bone－marrow
(contributed by Chris Jackson (chris.jackson@bris.ac.uk))A protocol I have used to isolate rat bone marrow is:?1. Kill the rat and dissect out the fem
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates4
The use of a micro-titre based colorimetric assay provided a third method for the study of ACTase activity, and the most useful for large scale measur
A－quick－method－to－isolate－plant
Use from 0.01 - 0.1 gram plant material. Grind the plant material with liq.N2 in a mortar.We normally use some alumina to crush hard tissue.
A－Method－for－Structure1
A Method for Structure–Activity Analysis of Quorum-Sensing Signaling Peptides from Naturally Transformable StreptococciMany species of streptococci se
A－Method－for－Structure2
CD SpectroscopyCD spectra for peptides and reference solutions were recorded at 298?K on a Jasco J-920 CD spectrometer with a 1-mm quartz cuvette. Spe
A－Method－for－Structure3
CSP-Dependent Transformation AssayTo determine if synthetic peptides activated quorum sensing for induction of genetic competence, we used the?comC?de
A－Method－for－Structure4
We also used this mutant (SMdC) as a host to generate two types of?lacZ?transcriptional reporter fusion strains to assay the promoter activity of QS-c
A－Method－for－Structure5
ConclusionsQuorum-sensing signaling systems involving the interaction between a signaling peptide and its cognate histidine kinase receptor are widely
Easy－YAC－Preparation－Method
YAC TRANSFORMATION OF C. ELEGANS USING TOTAL YEAST GENOMIC DNA[This method is described in The Worm Breeder's Gazette (1997) A. Davies and J. Shaw
Isolation－of－Functional－Photosystem－II－Core－Particles－From－the－Cyanoba...
This chapter contains the description of several methods used for the isolation of functional photosystem (PS)II core particles from wild-type (wt
ELISA
Gangliosides ELISA protocol?(Contributed by?pingsunjim)This protocol can be used for detection of gangliosides.Specific antibodies to gangliosides are
E.Z.N.A.?－Total－RNA－Midi－Kit－Protocol－DNase－I－digestion－Protocol
實驗概要E.Z.N.A.? ?Total RNA Midiprep Kit provides a rapid and easy method for the ?isolation of up to 600 ug of total RNA from cultured eukaryotic cells,
Purification－of－human－mononuclear－cells－and－neutrophils
PurposeMaterials10ml 6% dextran + 7ml citrate/citric acidDextran: T500 --> 6g+100ml PBSCitrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS4
DNA－Purification－from－Agarose－Gels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide. Locate bands with a hand-held long-wave UV lamp.2. Slice the gel wit
Isolation－of－colonic－epithelium
實驗概要The ?method we use is based on work of Dr. Hazel Cheng, at the University of ?Toronto and works for both colon and the small intestine.First ?we e
Poly(A)+RNA－Isolation
Eukaryotic messenger RNA (mRNA) can be separated from the other RNA species in a total RNA preparation by affinity chromatography by virtue of the
Isolation－of－papillary－cells
Isolation of renal papillary cells1.?For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
Isolation－of－liver－lymphocyte
Isolation of liver lymphocyte???Several lymphocyte subpopulations reside in the normal adult human liver. Liver lymphocytes mainly include a large n
Isolation－Of－PCR－Products
實驗概要Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents.實驗原理The ChargeSwitch? TechnologyT
Cosmid－DNA－Isolation
實驗概要Isolation of high yields of highly pure cosmid DNA using PureLink? HiPure Plasmid Purification Kits.實驗原理The ?PureLink? HiPure Plasmid Purification
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by k