Purificationofhumanmononuclearcellsandneutrophils
PurposeMaterials10ml 6% dextran + 7ml citrate/citric acidDextran: T500 --> 6g+100ml PBSCitrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS43 ml blood12 ml RT Histopaque 107718 ml cold H2O2 ml 10x PBSM199 for HUVECs: 1L powder pocket M199 + 35g NaHCO3 + 25mM Hepes + 5 mM glutamine + 50 ug/ml Gentamycin.1M Hepes: 119.1 g Hepes + 500 ml dH2O.Media A: 1X HBSS (10X:50ml) + 10 mM Hepes (1M: 5 ml) + 5 mM EDTA ......閱讀全文
Purification of human mononuclear cells and neutrophils
PurposeMaterials10ml 6% dextran + 7ml citrate/citric acidDextran: T500 --> 6g+100ml PBSCitrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS4
L-selectin-PNAd Interactions under Flow Conditions.
Purpose The main purpose of the flow chamber assay is to visualize and measure interactions between flowing cells expressing a given adhesion molecu
Human Peripheral Blood Mononuclear Cell Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained fr
Isolation of peripheral blood mononuclear cells (PBMC)
1.?Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2.?PBMC were isolated by centrifugation over lymphocyte separation medium. 3
Different types of human cells
Human primary cells are cells taken from living human beings and cultured.? These cells retain the differentiation of the original cells taken in
Isolation of human prostatic epithelial cells
1.?A small piece of tissue from each specimen was removed and minced. 2.?The tissue was digested with collagenase overnight. 3.?To remove the colla
Culturing Human Neural Stem Cells
實驗概要 Neural ?stem cells (NSC) are valuable resources because of their ability to ?differentiate into neurons and glial cells with applications in ?n
Isolation of human endometrial epithelial cells
Tissue collection1.?Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2.?All women reported
Isolation of Human Pulmonary Epithelial Cells
?Isolation of Human Pulmonary Epithelial Cells????1.?Pulmonary epithelial cells from human lung tissue were isolated.2.?Lungs were perfused with 10
Isolation of human atrial or ventricular cells
1.?Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.2.?Isolated myocardial tissue w