WIKI資訊
I. Purpose:A. Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected mosaicism, confirmation of a new chromosome disorder, or special dermatological disorders. Tissue may also be used when blood cultures are not available, such as on stillbirths. B. The skin biopsy should be performed by a physician, with scrupulous aseptic te......閱讀全文
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
實驗概要 AMNIOTIC FLUID CULTURES ON COVERSLIPS主要試劑Solutions:Colcemid working solution: 10 mcg/ml Colcemid in Hank's Balance
General StrategyWe typically work with tissue culture, primary mammalian cells, and cell extracts, but the protocols can be adapted to other systems,
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed CellsActin: Louise CramerTubulin: Arshad DesaiGeneral StrategyWe typically wor
I. Purpose:Amniotic fluid may be used for prenatal diagnosis of aneuploidy or other structural abnormalities. II. Culture Procedure:A. Aseptic te
LEVEL IIMaterialsCoverslip cultures of an appropriate monolayer cell linePhosphate buffered saline (PBS)Acetone/Methanol (absolute) in a 50:50 volume
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
常規操作(主要內容如下)· Aseptic Technique· Culture Ves
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
Critical step Be careful to thaw PNS and cytosol slowly on ice to avoid unwanted protein degradation or breaking of organelles. This might requir
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
Please note: lately (2005 - present), we normally culture PtK1 cells in F-12 media, but they also grow in other types of media as described below.Mixi
實驗概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples
實驗概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples
實驗概要The protocol provides a method of hippocampal neuron cultures.主要試劑Begin by timing the pregnant mouse at E17-E19 days of gestation. Have ready the
IntroductionLive imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It all
實驗概要A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic b
Introduction to Immunocytochemistry (House Ear Institute)A brief overview of common available methods. BrDU Immunocytochemistry using
Protocol for the Primary Culture of Cortical and Hippocampal neurons Solutions and media required:Poly D-lysine/laminin solution - pdfD
Full size image (70 KB)In vitro incubation of a reaction mix that contains labeled endosomes, cytosol and an ATP-regenerating system at phys
Small DNA-probes from cosmids or plasmids clonesThese kind of probes, especially plasmids, have become out of fashion for 3D-FISH due to their delicat
Primary cultures of intrahepatic bile duct epithelial cells isolated and cultured1. Liver was surgically removed and perfused via the hepatic vei