WIKI資訊
8 eggs per day, day 7- day 13 cut CAM, wash in precooled PBS,in 10 ml WASH 1 (PBS, 5 mM EDTA, COMPLETE) on icecut in pieces in petri dish on icecentrifuge 4 min at 1000 rpm (table top centifuge, Falcon 15 ml tubes) at 4°C (precool Heraeus centrifuge in E2)aspirate supernatant, add 10 ml WASH 2 (PBS, 5 mM EDTA, 5 μg/ml Leupeptin+ 0.5 mM Pefabloc SC)centrifuge 4 min at 1000 rpm (table top centifuge, falcon 15 ml t......閱讀全文
DescriptionThis is a fast and easy assay to test the angiogenic/anti-angiogenic properties of molecules. As compared to other angiogenesis assays, suc