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Phycoerythrin conjugation protocolDavid's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well.ReagentsAbAntibody, about 2.5 mg/mlPER-Phycoerythrin, purified (Molecular Probes P-801 in 60% ammonium sulfate)PBSPhosphate buffered saline without Ca2+/Mg2+MeOHMethanolDMSODimethyl sulphoxide, anhydrousSPDP3-(2-pyridyldithio)propionic acid N......閱讀全文
Conjugation of monoclonal antibodiesPrelude: You are free to copy and distribute these documents at will--but please do so in their entirety, complete
Biosynthetic labeling (Sefton Lab)Biotinylation of Antibody (Contributed by Nanci Donacki)125I Labeling of Protein using ICl (ScienceXc
最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯： 嗚咽分享到： Flow Cytometry Analysis (Springer Lab, Harvard University)Flo
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excit
Biotinylation of Antibody ( Contributed by Nanci Donacki) Antibody labeling (House Ear Institute)Labeling protocols using af