CrystalVioletAssay
This is a simple assay useful for obtaining quantitative information about the relative density of cells adhering to multi-well cluster dishes. The dye in this assay, crystal violet, stains DNA. Upon solubilization, the amount of dye taken up by the monolayer can be quantitated in a spectrophotometer or plate reader.Carefully remove culture medium from wells.Wash plate gently with PBS warmed at least to roo......閱讀全文
Crystal Violet Assay
This is a simple assay useful for obtaining quantitative information about the relative density of cells adhering to multi-well cluster dishes. The dy
Vybrant? DyeCycle? Violet Stain
實驗概要Live cell studies ?of cellular DNA content and cell cycle distribution are useful to detect ?variations of growth patterns due to a variety of phy
LIVE/DEAD? Violet Viability/Vitality Kit
實驗概要The ?LIVE/DEAD? Violet Viability/Vitality Kit provides a two-color ?fluorescence cell viability and vitality assay that is based on the ?simultane
LIVE/DEAD? Violet Viability/Vitality Kit
實驗概要The LIVE/DEAD? ?Violet Viability/Vitality Kit provides a two-color fluorescence cell ?viability and vitality assay that is based on the simultaneo
Gram-staining Procedure
Gram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should
Standard CellTrace? Violet T-Cell Procedure
實驗概要The CellTrace? ?Violet Cell Proliferation Kit provides a versatile and well-retained ?cell tracing reagent in a convenient and easy-to-use form. T
SOFT AGAR ASSAY FOR COLONY FORMATION
Note: All volumes are calculated to cater for four plates per point. Base Agar 1. Melt 1% Agar (DNA grade) in microwave, cool to 40 in a waterbath
Adhesion Assay Protocol
Materials to be prepared beforehand:1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI)2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI)3) Lamini
Pectinase assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are
Protease assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to soft