SREBPcontroloflipidsynthesis
Sterol-regulatory element binding proteins (SREBPs) play a key role in transcriptional regulation of cholesterol metabolism in response to cholesterol levels in the cell. When cholesterol is abundant in the cell, the SREBPs are retained in the ER. When cholesterol levels decrease, SREBPs are cleaved and released to act as transcription factors, binding to the promoters of genes such as the LDL receptor and HMG CoA Sy......閱讀全文
SREBP control of lipid synthesis
Sterol-regulatory element binding proteins (SREBPs) play a key role in transcriptional regulation of cholesterol metabolism in response to cholesterol
Regulation And Function Of ChREBP in Liver
Liver is the major site for carbohydrate metabolism (glycolysis and glycogen synthesis) and triglyceride synthesis (lipogenesis). While insulin was lo
LIPID EXTRACTION
PROCEDURE 1. Resuspend tissue/extract in 5 volumes of 1:1 chloroform/methanol by vortexing (to homogeneity or as close as possible) 2. Add t
Lipid analysis
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer
Lactose Synthesis
The synthesis of glycosidic bonds between monosaccharides requires energy input, energy that is provided by joining sugar monomers to a nucleotide suc
Synthesis of plasmalogens
Plasmalogens are a class of glycerophospholipids that contain one vinyl-ester linked long chain alcohol group in place of one ester-linked fatty acid
Sucrose Synthesis
The prodution of sucrose takes place in plants, to store energy produced during photosynthesis. The extraction of sucrose from plants like sugar cane
Starch Synthesis
The primary carbohydrate used for energy storage in plants is starch, the equivalent of glycogen in animals. Both starch and glycogen are polymers of
Pest Control
We use Dimilin G (4% diflubenzuron) from Maag AG to fight the small dipteran black flies (Trauermücken). It is a granular substance and sprinkled
Library cDNA Synthesis
Library cDNA Synthesis 1° cDNA Synthesis N.B:?During 1° cDNA synthesis, all steps should be carried while wearing gloves and all solutions should e