毛細管電泳及其應用(capillaryelectrophoresis,CE)
一、毛細管電泳的概述：毛細管電泳又稱高效毛細管電泳，它是在熔融的石英毛細管（內徑為25~100?m）中進行電泳，其管內填充緩沖液或凝膠，是近年來進展最快的分析方法之一。毛細管電泳是電泳技術和現代微柱分離相結合的產物，它具有效率更高、速度更快、樣品和試劑消耗量特少的特性。毛細管電泳儀的基本結構：1、 高壓電極槽與進樣機構 2、填灌清洗機構 3、毛細管 4、檢測器 5、鉑絲電極 6、低壓電極槽 7、恒溫機構 8、記錄/數據處理毛細管的結構：毛細管電泳通常使用內徑為25~100μm的彈性（聚酰亞胺）涂層熔融石英管。 毛細管的特點是：容積小；側面/截面積大而散熱快、可承受高電場；可使用自由溶液......閱讀全文
Chromatin－Electrophoresis
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise 10.4 - Chromatin Electrophoresis LEVEL II Materials ? 14 M Ure
Capillary－Electrophoresis
Capillary electrophoresis is a very sensitive analytical technique. Sample components are separated within a fused silica capillary using one of sever
electrophoresis－of－DNA
Agarose?Gel?Electroporesis?of?DNA Making?the?gel: 1.? Place?casting?platform?with?well?former?sideways?in?gel?stand?where?you?wish?to? pour?
RNA－Electrophoresis
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location of th
DNA－Electrophoresis
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. DNA is a negatively
Protein－Electrophoresis
DefinitionAmino acids, nucleotides, polypeptides, and other compounds in a colloidal state can be separated by the application of external voltages wh
RNA－gel－electrophoresis
MaterialsDEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37oC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide solutions should also be
RNA－gel－electrophoresis
實驗概要RNA gel electrophoresis主要試劑DEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37oC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide sol
Pulse－Field－Electrophoresis
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
Agarose－Gel－Electrophoresis
實驗概要Separating nucleic acid fragments by agarose gel electrophoresis.實驗原理?Agarose ?gel electrophoresis remains the most widely used technique for ?sep
Agarose－gel－electrophoresis
General ProcedureCast a gelPlace it in gel box in running bufferLoad samplesRun the gelImage the gelCasting Gels0.7% agarose gel with 1kbp ladder in U
Gel－Electrophoresis－of－DNA
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we
Standard－neutral－agarose－electrophoresis
Standard neutral agarose electrophoresis Standard agarose gels can be prepared using either TBE or TAE running buffers. You will need: Either 10 x
Agarose－Gel－Electrophoresis－of－DNA
1) Dissolve 1 g of agarose in 100 ml of 1X TAE or TBE buffer (gives a 1% gel). See note for making LMP agarose gel.?2) Cast the gel with the comb in p
ELECTROPHORESIS－OF－DNA－IN－AGAROSE－GELS
ELECTROPHORESIS OF DNA IN AGAROSE GELSA). AGAROSE CONCENTRATIONS:???????Use 0.8% agarose (w/v) for high molecular weight DNA fragments, and 1 - 1.2% f
Alkaline－agarose－gel－electrophoresis
Alkaline agarose gel electrophoresis (Sambrook et al., 1989)Alkaline agarose gels can be used to determine the size and quality of first and second st
ELECTROPHORESIS－OF－DNA－IN－POLYACRYLAMIDE－GELS
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall:???? ????????165 x 130 mmMedium: ????????165 x 200 mmLarge:??? ????????165 x 260 mm5% Anal
Blue－Native－Gel－Electrophoresis
Blue Native Gel ElectrophoresisStock solutions49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT3 x Gel buffer 150 m
Polyacrylamide－Gel－Electrophoresis－of－Oligonucleotides
1. Pour and polymerize a 20% polyacrylamide gel, no Urea.2. Remove clamps. Rinse with water. Remove comb. Rinse top of gel well.3. Insert comb teeth d
SDS－Gel－Electrophoresis－of－Tubulin\MAPs
MaterialsStock Acrylamide: (30%T:0.8%C)30% by weight of acrylamide0.8% by weight of N,N'-bis-methylene acrylamideSeparation Gel (Final Concentrati
High－Resolution－Agarose－Gel－Electrophoresis
實驗概要Agarose gel ?electrophoresis remains the most widely used technique for separating ?nucleic acid fragments due to its ease of use, non-toxicity, a
Denaturing－Gradient－Gel－Electrophoresis－(DGGE)
Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to h
Lipoprotein－Analysis－Week－2:－Electrophoresis
Lipoprotein Analysis??Week 2: Electrophoresis?IntroductionSDS polyacrylamide gel electrophoresis (SDS PAGE) will be used to assess the purification pr
Denaturing－Agarose－Gel－Electrophoresis－of－RNA
The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about R
2D－Polyacrylamide－Gel－Electrophoresis
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Electrophoresis－of－PCR－products－with－Sunrise－gel－apparatus
Electrophoresis of PCR products with Life Technologies Sunrise gel apparatusGel:?In a 500 ml Pyrex? glass bottle, add:Agarose:3 gH2O270 mls10X TA30 ml
Top－10－Fun－Facts－for－DNA－Electrophoresis
Did you know:When preparing agarose for electrophoresis, it is best to sprinkle the agarose into room-temperature buffer, swirl, and let sit at least
Native－gel－electrophoresis（非變性電泳）
Native gel electrophoresis?Under native PAGE conditions, polypeptides retain their higher-order structure and often retain enzymatic activity and inte
Lipoprotein－Analysis－Week－2:－Electrophoresis2
Preparation of stacking gelPrepare a 7.5 ml of 3% stacking gel in a small beaker using the following amounts of appropriate reagents.Stockfinal conc.A
QUALITATIVE－ANALYSIS－OF－DNA－FRAGMENTATION－BY－AGAROSE－GEL－ELECTROPHORESIS
1. IntroductionNuclear morphology changes characteristic of apoptosis appear within the cell together with a distinctive biochemical event: the endonu