EffectofCyclopamineonXenopuslaevisembryos
Cyclopamine and jervine, teratogens derived from the skunk cabbage Veratrum californicum, are known to induce holoprosencephaly in chick embryos when introduced prior to gastrulation. Holoprosencephaly, a condition characterized by the reduction or apparent absence of left-right separation in certain tissues of the embryo, can be produced by a deficiency in the Sonic hedgehog signaling system. This disorder, als......閱讀全文
Effect－of－Cyclopamine－on－Xenopus－laevis－embryos
Cyclopamine and jervine, teratogens derived from the skunk cabbage?Veratrum californicum, are known to induce holoprosencephaly in chick embryos when
Investigating－the－effect－ofthe－teratogen－cyclopamine－on－Ambystoma－mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
Whole－mount－TUNEL－analysis－of－Xenopus－embryos
Fixation and pretreatmentDejelly albino embryos carefully in 2% Cystein (pH 7.8).Remove the vitellin membrane with two pairs of tweezers???????? (or c
Cell－death－detection－in－Xenopus－embryos－by－ELISA
Sample preparationWash embryos 1 x in 25% MMRRemove excess bufferAdd 10 volumes "incubation buffer", i.e. 50 μl for 5 embryosLyse the embryos by?gentl
DEVELOPMENTAL－EFFECTS－OF－LITHIUM－CHLORIDE－ON－XENOPUS－EMBRYOS
PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula?xenopus?embryos.INTRODUCTION: When th
The－effect－of－lithium－chloride－on－Lytechinus－variegates－embryos
PurposeThis experiment will study effects of lithium chloride on sea urchin development, forcusing on archenteron formation.IntroductionGastrulation i
Effect－of－UV－Treatment－on－Early－Development－of－Sea－Urchin－Embryos
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
The－Effect－of－Caffeine－on－the－Heart－Rates－of－ThreeDay－Old－Chick－Embryos
Objective??????The purpose of this experiment is to observe the effect of caffeine on the heart rate of three day old chick embryos by directly treati
The－effects－ofdifferent－concentrations－oflithium－chloride－on－thedevelopment
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular?Brachydanio rerio?embryos results in
發育生物學
In Vitro Production of Bovine Embryos?(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)This protocol describe procedures for in vitro
Dorsal－Lip－Transplant－in－Amphibian－Embryos
IntroductionIn early stages of development in amphibian embryos, very few cells' fates are rigidly determined. However, previous experiments show
Embryo－Dissection－and－Micromanipulation－Tools
Embryo Dissection and Micromanipulation ToolsHazel L. Sive,?Robert M. Grainger, and?Richard M. HarlandAdapted from "Equipment for Embryo Experiments,"
FGFR－INHIBITOR,－SU5402,－ON－CRANIOFACIAL－DEVELOPMENT－IN－CHICKEN－EMBRYOS
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
基質效應（matrix－effect）
化學分析中，基質指的是樣品中被分析物以外的組分。基質常常對分析物的分析過程有顯著的干擾，并影響分析結果的準確性。例如，溶液的離子強度會對分析物活度系數有影響，這些影響和干擾被稱為基質效應（matrix effect）。什么是基質效應？基質是指的是樣品中被分析物以外的組分。基質常常對分析物的分析過程有
SingleCell－Electroporation－in－Xenopus
Single-Cell Electroporation in XenopusXue Feng Liu and Kurt HaasINTRODUCTIONSingle-cell electroporation (SCE) is a versatile technique for delivering
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Fixation－of－Embryos
MEMFA Fix10xMEMFA Salts1 part 10x MEMFA salts1 M MOPS1 part 37% formaldehyde20mM EGTA8 parts water10mM MgSO410x salts can be autoclaved and stored. Tu
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
Isolation－of－mouse－embryos
1. Sacrifice impregnated mouse.2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps,use another to tear the mesome
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by k
Wholemount－staining－of－embryos
Fix embryos in formalin or MEMFA for one hour at room temperature with mixing. Rinse with TBS, replace with methanol, store at -20oC.Rehydrate by slow
The－effect－of－temperature－on－the－embryonic－development－of－sea－urchins
Objective:?This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the external en
The－Effect－of－pH－Levels－By－Varying－the－Acidity－of－theExtracellular－Solution
BackgroundZebrafish,?Brachydanio rerio, have a distinct embryonic development. The embryo goes through a process?called epiboly. Epiboly, as defined i
Sectioning－stained－embryos.
The protocols for plastic and wax sections as used by the Vize lab. These protocols work, but they have not been optimized. If anyone has better proto
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution:??Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
Obtaining－Embryos:－hCG－Injection
Ovulation is induced in X. tropicalis by injection of human chorionic gonadotropin (hCG). X. tropicalis requires a much smaller dose than does X. laev
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution:??Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
In－vitroculture－of－early－chick－embryos
1. Use sterile technique. Prewarm Howard's Ringers in petri dish andagar/albumin culture dish to 37oC.2. Crack 2-day egg into large sterile petri
inner－filter－effect－是什么意思
inner filter effect內濾波效應；內濾效應；內部濾光效應
關于真核生物的基因調控—基因擴增的介紹
另一種改變基因數量而調節基因表達的方式稱為基因擴增。基因擴增是細胞短期內大量產生出某一基因拷貝的一種非常手段。某些脊椎動物和昆蟲的卵母細胞能夠專一性地增加編碼核糖體RNA的DNA(rDNA）序列。例如非洲爪蟾(Xenopus laevis）的卵母細胞中的rDNA的拷貝數可由平時的 1500急劇增