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Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural TransformationInactivation of a chromosomal gene is a useful approach to study the function of the gene in question and can be used to produce a desired phenotype in the organism. This chapter describes how to generate such mutants of the cyanoba......閱讀全文
FL3500雙調制葉綠素熒光儀 （新升級型號為FL6000） FL3500雙調制葉綠素熒光儀是專門用于對藍綠藻或綠藻等微藻，葉綠體或類囊體懸浮物，乃至葉片進行光合作用研究的強大科研工具。儀器具備雙通道測量控制，可控制測量樣品的溫度，并配備單翻轉光（STF），內置多種可用戶自行修改的測量程序
FMT150藻類培養與在線監測系統將生物反應器與監測儀器獨特地結合在一起，用于淡水、海水藻類和藍細菌（藍藻）等的模塊化精確光照培養與生理監測。FMT150可以通過控制單元（包括電腦與預裝軟件，軟件分為基本版與高級版）中用戶自定義程序動態自動改變培養條件并實時在線監測培養條件與測量參數。光強、光質、
BRCA1 and BRCA2 were identified genetically as breast cancer susceptibility genes when a single copy of the gene is mutated and are involved in the ce
2011CJ Bernacchi, ADB Leakey, BA Kimball, DR Ort (2011) Growth of soybean at future tropospheric ozone concentrations decreases canopy evapotransp
截至2019年12月23日，中國學者在Cell，Nature及Science在線發表了107篇文章（2019年的Cell ，Nature 及Science 已經全部更新），iNature團隊對于這些文章做了系統的總結： 按雜志來劃分：Cell 發表了31篇，Nature 發表了44篇，Scie
E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo
G. Bacterial cell maintenanceFour strains of E. coli are used in these studies: JM101 for M13 infection and isolation (4), XL1BMRF' (Str
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
We also used this mutant (SMdC) as a host to generate two types of lacZ transcriptional reporter fusion strains to assay the promoter activi
CD SpectroscopyCD spectra for peptides and reference solutions were recorded at 298 K on a Jasco J-920 CD spectrometer with a 1-mm quartz cuvette
5月29日下午，上海交大生命學院召開推進學院文化建設動員會。學院院長鄧子新院士在講話中，就如何開展實驗室文化建設和學院教師分享了他多年的心得。鄧院士認為，學校開展校園精神文化建設，生命學院應趁勢而上，積極推進學院精神文化建設，無論把這個活動納入全國的大框架，還是小到一個研究組，一個辦公室，都是一個及
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
CSP-Dependent Transformation AssayTo determine if synthetic peptides activated quorum sensing for induction of genetic competence, we used the co
三、肝臟AAV2、AAV5、AAV7 和 AAV8，其中 AAV7 和 AAV8 的效率是 AAV2 的 10-100 倍。注射部位常選擇門靜脈、外周靜脈和尾靜脈。注射病毒滴度一般選擇 8×10^10-2×10^12(GC/kg)（人、靈長類）；1×10^11 GC/只鼠，稀釋成 50-10
Homeodomain transcription factors comprise a large family of DNA binding factors that regulate transcription and development. Many homeodomain genes a
A Method for Structure–Activity Analysis of Quorum-Sensing Signaling Peptides from Naturally Transformable StreptococciMany species of streptococci se
Purification of Kar3 Motor Domain ProteinMaterialsInduced cells (2 - 5 g pellet of pET/Kar3 in BL31(DE3)pLysS host cells) (See note #1)HEM b
John “JC” Carver, a payload integration engineer with NASA Kennedy Space Center’s Test and Operations Support Contract, uses a FluorPen to measure
Assay of superoxide dismutase activity by combining electrophoresis and densitometryAbstract. A modified technique was developed to assay superoxide d
實驗概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
Mitogen-activated protein (MAP) kinases are important players in signal transduction pathways activated by a range of stimuli and mediate a number of
The differentiation of muscle cells is transcriptionally regulated, in part by the myocyte enhancer factor-2, MEF2. During myogenesis MEF2 binds to My
The signal transduction cascade responsible for sensing light in vertebrates is one of the best studied signal transduction processes, and is initiate
【摘要】 目的：探討堿性成纖維細胞生長因子(bFGF)與膀胱癌發生發展的關系。方法：應用免疫組化方法檢測了48例膀胱癌存檔標本和4例正常膀胱組織中bFGF的蛋白表達。結果：48例膀胱癌存檔蠟塊中有23例陽性，浸潤性癌顯著高于淺表癌(P＜0.01)，除G1和G2之間外各分級陽性率之間差異也有顯著性。陽
一、會議內容安排 報到日期：2014年10月25日，下午2:00-8:00pm 報到地點：貴州大廈 會議日期：2014年10月26-27日 會議地點：北京化工大學會議中心 住宿地點：北京化工大學招待所 櫻花賓館 如家快捷酒店 貴
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive
C. Restriction digestionRestriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restrict
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
This chapter contains the description of several methods used for the isolation of functional photosystem (PS)II core particles from wild-type (wt