Mesenchymalprogenitorcellsderivedfromhumanmuscle
Harvesting traumatized muscle derived mesenchymal progenitor cells (MPCs)1. The muscle tissue was dissected without contaminating by granulation, adipose or fibrous tissues.2. The excised muscle was transferred into a dish containing primary cell culture system and minced until the slurry could easily passed through the tip of a 25 mL serological pipette.3. The minced muscle tissue was then tra......閱讀全文
Mesenchymal－progenitor－cells－derived－from－human－muscle
Harvesting traumatized muscle derived mesenchymal progenitor cells (MPCs)1.?The muscle tissue was dissected without contaminating by granulation, adip
Isolation－of－human－multipotent－mesenchymal－stem－cells－from－second
Isolation of human multipotent mesenchymal stem cells from second‐trimester amniotic fluid?Culture of MSC from amniotic fluid1.?Twenty amniotic flui
Culture－of－human－venous－smooth－muscle－cells
Culture of human venous smooth muscle cells???1.?Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared
Isolation－of－human－uterine－smooth－muscle－cells
1.?Approval to utilize human tissue was given by Hospital's Committee on Research Involving Human Subjects. 2.?Human myometrial and leiomyoma tiss
Isolation－of－human－prostatic－smooth－muscle－cells
Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of the prostate. 2.All
Isolation－and－Culture－of－human－vascular－smooth－muscle－cells
1.?Human vascular smooth muscle cells?(SMCs) were isolated from human saphenous vein.2.?Small moistened pieces of tissue (6 mm2) were placed intima
GentleMACS/單細胞懸液制備文獻集錦
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? ? ? ? ? ? 種屬 處理組織 文章名稱 human ? ? ? ? ? ? ?
Ex－Vivo－Human－Primary－Mesenchymal－Stem－Cells－(MSCs)－Culture
Human bone marrow contains mesenchymal progenitors (mesenchymal stem cells, MSCs).? MSCs produce adventitial cells in the human bone marrow microenvir
Isolation－and－culture－of－human－pulmonary－artery－smooth－muscle－cells－...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs)1.?The human pulmonary arteries were opened to expose the endothelial su
Derivation－of－Dopaminergic－Neurons－(from－Human－Embryonic－Stem－Cells)
實驗概要Directed ?differentiation of specific lineages has been a focal point in the ?field of human embryonic stem cell (hESC) research. Cell replacement
Isolation－of－stromal－vascular－cells－from－human－adipose－tissue
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
CD133－isolation,－expansion,－and－differentiation
1.?Renal progenitor cells were obtained from the normal portion of cortex obtained from surgically removed kidneys. 2.?After dissection and passage th
Isolation－and－Culture－of－Multipotent－Stem－Cells－from－Human－Bone－Morrow
Bone marrow contains three types of stem cells:1.?Hematopoietic stem cells give rise to the three classes of blood cells that are found in the circu
Isolation－and－cultivation－of－endothelial－progenitor－cells－(EPCs)
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
Peripheral－blood－“endothelial－progenitor－cells”
EPC Isolation and Characterization1.?EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton?and?Larysa H. Pevny1UNC Neuroscience Center
胚胎干細胞和成體干細胞標志物
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
Preparation－of－Conventional－Actin－from－Skeletal－Muscle
Modified from Spudich & Watt, 1971, JBC 246:4866.1. Mix 20 ml buffer G with each gm of muscle acetone powder. Extract with stirring on ice for 30 min.
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（二）
?Figure 2: Verification of C1 Single-Cell mRNA-Seq data quality. a)ERCC ?RNA Spike-In Control Mix 1 was applied to a C1 IFC at a total ?transcript in
ex－vivo－expanded－endothelial－progenitor－cells
Cell Culture. 1.?Total hPBMCs were isolated from blood of human volunteers by density gradient centrifugation. 2.?Cells were plated on culture dishes
PriCells:－Isolation－of－endothelial－progenitor－cells－(EPCs)
?PriCells: Isolation of endothelial progenitor cells (EPCs)???1.?Twenty-four mL venous blood was collected at each time point into Vacutainer CPT Mono
Isolation－of－human－primary－airway－smooth－muscle
1.?Primary cultures of human primary airway smooth muscle (ASM) cells were prepared from explants of airway smooth muscle. 2.?The human trachea tiss
Decontamination－of－cells－from－the－yeast
I???? Destroy yeast1.???? Aspirate medium and wash cell in PBS.2.???? Incubate cells at 37oC for 5 min in non-diluted antibiotic-antimycotic.3.???? In
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells2
Slide Coating13. Just before use, dilute the poly-D-lysine stock solution?1:50 in H2O. Add 0.2 mL of diluted poly-D-lysine to each well?of an eight-we
Comparison－of－Enzymatic－and－NonEnzymatic－Means1
Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem CellsThe dissociation of adherent mesenchymal
Different－types－of－human－cells
Human primary cells are cells taken from living human beings and cultured.? These cells retain the differentiation of the original cells taken in
Isolation－and－Differentiation－of－AmnionDerived－Stem－Cells－(ADSCs)
ADSC isolation, culture, and cloningRat amnion membrane is mechanically separated from the chorion of embryonic day 18.5 Sprague–Dawley rat embryosThe
Primary－Culture－of－Pulmonary－Arterial－Smooth－Muscle－Cells
Primary Culture of Pulmonary Arterial Smooth Muscle Cells?1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from hum
Removing－cells－from－liquid－nitrogen
Put cryovial straight from storage and float in the 37篊 water bath- caution should be taken as on rare occasions vials can explode when heated up due
Harvesting－Hematopoietic－Cells－from－Mice
Materials4 mice from each genotype4 Ly5 miceBuckets with wet ice 3xBucket with dry ice 1xDewar flask with liquid nitrogen100 mL beakers with 95% ethan