免疫球蛋白提取技術(Immunoglobulinisolationtechnique)2
九、蛋白定量技術 （一）雙縮脲測定法 1．原理 蛋白質中的肽鍵有雙縮脲反應，在堿性溶液中與二價銅離子形成藍紫色的絡合物，在一定的范圍內，顏色的深淺與蛋白質的含量成正比。此法特異性強，游離的氨基酸、小肽和核酸均不產生這種反應，但此法不夠敏感，僅能測出毫克水平。 2．試劑配制 硫酸銅（CuSO4·5H2O） 1.50g 酒石酸鉀鈉 ......閱讀全文
Isolation－of－papillary－cells
Isolation of renal papillary cells1.?For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
DNA－isolation－extraction
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolati
Isolation－of－murine－splenocytes
Overview In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the c
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by keeping
Isolation－of－mouse－embryos
1. Sacrifice impregnated mouse. 2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps, use another to tear the mes
Isolation－of－colonic－epithelium
實驗概要 The ?method we use is based on work of Dr. Hazel Cheng, at the University of ?Toronto and works for both colon and the small intestine. First
Chromosomal－DNA－Isolation
Chromosomal DNA Isolation METHOD: Grow cells in 2-5 ml broth to late log phase. Pellet 1-2 ml cells in microfuge. Resuspend cells in 400
Cosmid－DNA－Isolation
實驗概要 Isolation of high yields of highly pure cosmid DNA using PureLink? HiPure Plasmid Purification Kits. 實驗原理 The ?PureLink? HiPure Plasmid Purif
Isolation－of－papillary－cells
實驗概要 This protocols provides a general protocol for isolation of papillary cells. 實驗步驟 Isolation of renal papillary cells 1.?For ?isolation of pa
Isolation－Of－PCR－Products
實驗概要 Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents. 實驗原理 The ChargeSwitch? Tech
mice－islet－isolation
1.?Islets of Langerhans were isolated from 5- to 7-week-old nonobese diabetic (NOD) mice. 2.?Which involves cannulation of the common bile duct and
RNA－Isolation－Protocol
RNA Isolation Protocol(Revised 5-15-2003)Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109?cells (OD600= 0.2 Dilute cells or scale up)
RNA－isolation－for－Microarray
Description?RNA extraction using TRI REAGENT. This method gives ample amout of RNA.Procedure?It is 3 days procedure.Day 1:1. Harvest the cells and cen
Arabidopsis－gDNA－isolation
This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only us
Isolation－of－liver－lymphocyte
Isolation of liver lymphocyte???Several lymphocyte subpopulations reside in the normal adult human liver. Liver lymphocytes mainly include a large n
Isolation－of－bone－marrow
(contributed by Chris Jackson (chris.jackson@bris.ac.uk))A protocol I have used to isolate rat bone marrow is:?1. Kill the rat and dissect out the fem
Fungal－DNA－Isolation
Fungal DNA IsolationSaghai-Maroof MA, Soliman KM, Jorgensen RA, & Allard RW (1984) PNAS 81:8014-8018DNA successfully isolated from fungal species of C
Nucleolar－Isolation－Protocol
We recommend that you first download and read this page as a?PDF file. Using that as your guide, you can then follow the protocol below and view a Qui
Yeast－Nuclei－Isolation
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
Poly(A)+RNA－Isolation
Eukaryotic messenger RNA (mRNA) can be separated from the other RNA species in a total RNA preparation by affinity chromatography by virtue of the
Isolation－of－colonic－epithelium
The method we use is based on work of Dr. Hazel Cheng, at the University of Toronto and works for both colon and the small intestine.First we excise t
RNA－Isolation－Protocol
Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagen
Isolation－of－lymphatic－endothelial－cells
Dermal Cell Suspensions??????????? 1.?Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery.
Plasmid－isolation－from－yeast
Pick colonies into 0.5ml of SD-Leu (or other appropriate SD medium) Vortex for 1min Leave to grow O/N for 18-24h at 30°C, 230-250rpm (best in 5ml b
Isolation－of－Microtubules－(Bovine－Brain)
LEVEL II Materials Freshly removed bovine brain?2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphi
T－and－B－cell－Isolation
Reagents? Heparin - 1000 U/ml? Ficoll-Hypaque? PBS? RPMI-1640 supplemented with 10 mM glutamine and 15% FBS? AET (0.14M) Dissolve 1.967 g AET in 35 m
ISOLATION－OF－RNA－FROM－BACTEROIDS
3 g nodules (fresh or frozen in liquid N2) were ground to a powder in mortar and pestle with liquid N2. To the powder was added ice cold 0.5 M mannit
Lipoprotein－Isolation－－First－week
As insects have an open circulatory system, the hemolymph can be simply collected through an incision in the body wall. Most conveniently, you should
Keratocyte－isolation－(corneal－fibroblasts)
Corneal keratocytes (corneal fibroblasts) that this corneal layer is specialized fibroblasts residing in the stroma, representing about 85-90% of
Cajal－Body－Isolation－Protocol
Buffers and solutions(All solutions are supplemented with Complete Protease inhibitor tablet (Roche, Cat no: 1-873-580) at the final concentration of