Isolationofmurinesplenocytes
OverviewIn order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cells can be manipulated ex vivo easily. This protocol suggests ways in which you can do this without a lot of equipment or expensive supplies. This protocol can also be used to make cell suspensions from other lymphoid organs, such as the thymus or lymph nodes (see Current......閱讀全文
Isolation－of－murine－splenocytes
OverviewIn order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cell
Analysis－of－murine－BM
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis.?Techniques to do this are well established in huma
Murine－Skin－Transplantation
Prior to the initiation of experiment:Obtain IACUC approvalSterilize surgical instruments and Q-tipsI. Harvest Donor TissueEuthanize donor mouse (in a
Th9－Polarization－of－Mouse－Splenocytes
實驗概要Th9 ?cells are a subpopulation of T-helper cells, characterized to be ?involved in allergic diseases and resistance against intestinal ?nematodes.
Preparation－of－Low－Density,－CollagenaseDigested－Splenocytes
1. Dilute 2 ml of 4000 U/ml Collagenase D as follows: 1 ml into 9 ml HBSS/Ca++/Mg++ (=400U/ml) and 1 ml into 39 ml HBSS/Ca++/Mg++ (=100U/ml). Put on i
immunofluorescence－of－rabbit－antimurine－RELMβ－by－Peprotech
實驗概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMβ by Peprotech.實驗步驟The following protocol used B6 mice
immunofluorescence－of－rabbit－antimurine－VEGF－by－Peprotech
實驗概要The following protocol provides a method of immunofluorescence of rabbit anti-murine VEGF by Peprotech.實驗步驟The following protocol used normal muri
immunofluorescence－of－rabbit－antimurine－RELMα－by－Peprotech
實驗概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMα by Peprotech.實驗步驟The following protocol used B6 mice
immunofluorescence－of－rabbit－antimurine－leptin－by－Peprotech
實驗概要The following protocol provides a method of immunofluorescence of rabbit anti-murine leptin by Peprotech.實驗步驟The following protocol used Trichur
Analysis－of－murine－BM:－specimen－choice,－sampling－and－processing.
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis.?Techniques to do this are well established in huma
Isolation－of－colonic－epithelium
實驗概要The ?method we use is based on work of Dr. Hazel Cheng, at the University of ?Toronto and works for both colon and the small intestine.First ?we e
Poly(A)+RNA－Isolation
Eukaryotic messenger RNA (mRNA) can be separated from the other RNA species in a total RNA preparation by affinity chromatography by virtue of the
Isolation－of－papillary－cells
Isolation of renal papillary cells1.?For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
Isolation－of－liver－lymphocyte
Isolation of liver lymphocyte???Several lymphocyte subpopulations reside in the normal adult human liver. Liver lymphocytes mainly include a large n
mice－islet－isolation
1.?Islets of Langerhans were isolated from 5- to 7-week-old nonobese diabetic (NOD) mice. 2.?Which involves cannulation of the common bile duct and
Isolation－Of－PCR－Products
實驗概要Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents.實驗原理The ChargeSwitch? TechnologyT
Cosmid－DNA－Isolation
實驗概要Isolation of high yields of highly pure cosmid DNA using PureLink? HiPure Plasmid Purification Kits.實驗原理The ?PureLink? HiPure Plasmid Purification
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by k
Isolation－of－mouse－embryos
1. Sacrifice impregnated mouse.2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps,use another to tear the mesome
Yeast－Nuclei－Isolation
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
RNA－isolation－for－Microarray
Description?RNA extraction using TRI REAGENT. This method gives ample amout of RNA.Procedure?It is 3 days procedure.Day 1:1. Harvest the cells and cen
Isolation－of－papillary－cells
實驗概要This protocols provides a general protocol for isolation of papillary cells.實驗步驟Isolation of renal papillary cells1.?For ?isolation of papillary c
Isolation－of－colonic－epithelium
The method we use is based on work of Dr. Hazel Cheng, at the University of Toronto and works for both colon and the small intestine.First we excise t
Isolation－of－bone－marrow
(contributed by Chris Jackson (chris.jackson@bris.ac.uk))A protocol I have used to isolate rat bone marrow is:?1. Kill the rat and dissect out the fem
RNA－Isolation－Protocol
Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagen
RNA－Isolation－Protocol
RNA Isolation Protocol(Revised 5-15-2003)Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109?cells (OD600= 0.2 Dilute cells or scale up)
Chromosomal－DNA－Isolation
Chromosomal DNA IsolationMETHOD:Grow cells in 2-5 ml broth to late log phase.Pellet 1-2 ml cells in microfuge.Resuspend cells in 400 μl TES (50 mM Tri
Arabidopsis－gDNA－isolation
This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only us
Fungal－DNA－Isolation
Fungal DNA IsolationSaghai-Maroof MA, Soliman KM, Jorgensen RA, & Allard RW (1984) PNAS 81:8014-8018DNA successfully isolated from fungal species of C
Nucleolar－Isolation－Protocol
We recommend that you first download and read this page as a?PDF file. Using that as your guide, you can then follow the protocol below and view a Qui