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Lipoprotein Analysis Week 2: Electrophoresis IntroductionSDS polyacrylamide gel electrophoresis (SDS PAGE) will be used to assess the purification process and to determine the apparent molecular weights of the three apoproteins.SDS-Polyacrylamide gel electrophoresisElectrophoresis is the process in which charged particles migrate through a solid or liquid matrix in response to application of an electr......閱讀全文
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of
1,關于使用三氯醋酸沉淀法后蛋白質難于重懸的問題Q:1,Hi !I have been trying to clean and concentrate proteins form eucariotic cell culture medium in order to analyse them in 2
2013年5月16日-19日，由中國化學會主辦、廈門大學承辦、復旦大學、浙江大學協辦的為期四天的第八屆全國微全分析系統學術會議、第三屆全國微納尺度生物分離分析學術會議暨第五屆國際微化學與微系統學術會議在美麗的海濱城市廈門隆重召開。以下是微全分析專場報告。上海交通大學
尊敬的各位同行： 第2 屆大連國際色譜學術報告會及儀器展覽會包含第37屆國際高效液相色譜及相關技術會議(含儀器展覽會) (HPLC 2011 Dalian) 及第18屆全國色譜學術報告會及儀器展覽會(18th NSEC)，將于2011年10月8-11日在大連召開。 為使更多色譜工作者
Preparation of protein samplesIntracellular virus proteinsThe following method has been developed principally for the analysis of intracellular protei
METHOD Analysis of precipitated chromatin fractions (from Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analy
INTRODUCTION After chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is p
芯片毛細管電泳具有進樣量少，靈敏度高，分析速度快等特點，非常適合法醫DNA-STR的快速檢驗。毛細管電泳芯片由于尺寸小，可施加較大場強，所以在幾秒鐘內就可完成對樣品的分離，微陣列毛細管電泳芯片可實現高通量檢測則成為目前學者研究的熱點。2002年Emrich CA等[31]報道的將高通量384孔毛
ADSC isolation, culture, and cloningRat amnion membrane is mechanically separated from the chorion of embryonic day 18.5 Sprague–Dawley rat embryosThe
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
3. Commentary 3.1. Background informationApoptosis is an innate mechanism of eukariotic cell suicide which plays a major role i
③蛋白質分析 Duffy等［16］利用CD盤式塑料陣列芯片采用離心的方式進行了堿性磷酸酶分析，每個樣品檢測只需3mL試劑，幾分鐘內可分析幾十個樣品。瑞典的GYROS公司已生產出類似的產品并進行了肌球蛋白、IgG、IgA分析［17］。近來Burke 和Regnier［18］在芯片上利用電
Randomly Amplified Polymorphic DNA (RAPD)Randomly Amplified Polymorphic DNA (RAPD) by (DNA KAFFE)RAPD analysis has been successfully u
蛋白質電泳（主要內容如下）One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel Gel StainingRecipesOne-Dimensional SDS-PAGE·&
if mouse is found deadif mouse is a newborn or < 1 wk of ageIf mouse is alive or moribund and above one week of ageRoutine dissectionFormalin fixat
生物分子發表的代表性文獻 QTRAP：同時具有三重四極桿和線性離子阱性能的獨一無二的LC/MS/MS系統 QTRAP系統最早在ASMS 2002上，作為第一臺商用的線性離子阱發布，是世界上唯一的線性離子阱和三重四極桿的復合
A. Preparation of DNA SolutionIn the case of rice, for example This method may be appllicable for many grass species and some other plants. &n
［摘 要］ 目的：對中生北控生物科技股份有限公司生產的載脂蛋白AI、B液體雙試劑的性能進行評價。方法：采用中生載脂蛋白AI、B液體雙試劑，用免疫透射比濁法測定質控血清和住院病人血清載脂蛋白AI（ApoAI）及載脂蛋白B(ApoB)，分析中生載脂蛋白AI、B液體雙試
Evaluation of a Capability for The Liquid Double Reagent Assay Produced By The Zhongsheng Beikong Biotechnology and Science Inc For determine Lipo
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
毛細管電色譜的應用及發展毛細管電色譜(Capillary electrochromatography, 簡稱 CEC)是在毛細管中填充或在管壁涂布、鍵合液相色譜的固定相,然后在毛細管的兩端施加高壓直流電，在電場作用下產生電滲流(Electroosmotic f
在陸地生態系統中，在土壤中生活有數量龐大的微生物種群，包括原核微生物如細菌、藍細菌、放線菌及超顯微結構微生物, 以及真核生物如真菌、藻類( 藍藻除外) 、地衣等。它們與植物和動物有著明確的分工，主要扮演“分解者”的角色，幾乎參與土壤中一切生物和生物化學反應，擔負著地球C、N、P、S 等物質循環的
Methylation Specific PCRProtocol written by James Herman*Methylation Specific PCR (MSP) is a simple rapid and inexpensive method to determine the meth
1. 前言提取蛋白質是蛋白質組學研究的第一步。植物組織的蛋白質含量較低，并且存在多種非蛋白質成分，如細胞壁及貯藏多糖、脂質和酚類化合物，使蛋白質的提取難度增大 。植物蛋白質的可溶性與它們的細胞內定位關系密切，傳統的蛋白質提取方法是用水合緩沖液、去垢劑或直接沉淀法 [1] 。除了普遍使用的 TCA
Phosphoamino acid analysis:Mark Kamps's method1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electr
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
1. IntroductionNuclear morphology changes characteristic of apoptosis appear within the cell together with a distinctive biochemical event: the endonu
3. Characterization of the method precision and repeatability.a. Ensemble PCRs in the same conditions established before using quantities of
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o