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ReagentsCold absolute ethanol.0.5 M Na citrate stock (filtered), 50mM diluted stock.10 mg/ml RNase A (Boil 10 mins, cool, filter and store at -20°C).4 mg/ml Propidium iodide (PI) (filter and store in dark at -20°C). ORSYTOX Green (Molecular Probes catalog S-7020; 5mM stock in DMSO and stored in dark at -20°C)ProtocolSpin down 107 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour o......閱讀全文
最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯： 嗚咽分享到： Flow Cytometry Analysis (Springer Lab, Harvard University)Flo
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excit
Genome-wide Gene Expression Analysis (Richard Young Research Group,Whitehead Institute for Biomedical Research)A genoe-wide gene expression analy