WIKI資訊
I. ProcedureHarvest cells from tissue, preparing a single cell suspension. Red blood cells may be removed by lysis or density gradient: Red blood cells from murine peripheral blood or a spleen cell suspension can be lysed using BD Biosciences Pharmingen's PharM Lyse? (Cat. No. 35221E) solution. Add 2.0 ml of 1X Lysing Solution to the spleen cell suspension or per 200 μl of murine peripheral blood. Gently vortex i......閱讀全文
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Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excit