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實驗步驟Mice：8-16 weeks old malePrewarm buffer to room temperatureBuffer A : Ca2 /Mg2 ‐free Hank’s buffered saline solution [HBSS; Invitrogen, Grand Island, NY] containing 0.1% bovine serum albumin and 5 mM HEPES.Percoll stock solution: the stock Percoll solution is made by adding 1 ml of 1.08 M NaCl to per 7 ml Percoll; store at 4 degree for less then 3 months.81, 62, 55, 50, and 45% Percoll solutions:......閱讀全文
實驗概要Preparation of Mouse Neutrophils實驗步驟Mice：8-16 weeks old malePrewarm buffer to room temperatureBuffer A : Ca2 /Mg2 ‐free Hank’s buffered sali
Phycoerythrin conjugation protocolDavid's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal an