FungalGenomicDNAExtraction
OverviewHigh throughput of many fungal isolates can be achieved by growing axenic cultures in either (a) 1.5mL microfuge tubes, half full with liquid media (500uL), with a hole punched through the top for aeration; or if greater volumes are required, (b) peteri plates containing 10mL liquid media.In both cases, media is decanted and several water washes done to removemedia carbohydrates.ProcedureIf using petri plates......閱讀全文
Fungal Genomic DNA Extraction
Overview High throughput of many fungal isolates can be achieved by growing axenic cultures in either (a) 1.5mL microfuge tubes, half full with liqu
Fungal Genomic DNA Extraction
Overview High throughput of many fungal isolates can be achieved by growing axenic cultures in either (a) 1.5mL microfuge tubes, half full with liqu
Fungal Genomic DNA Extraction
實驗概要 This procedure does not require phenol extraction. The DNA is pure enough for restriction digests, PCR and genomic library construction. Hi
Automated Genomic DNA Extraction
實驗概要This section ?provides a general protocol for automated isolation of genomic DNA from ?10-20 μl blood samples in a 96-well format using the Charge
Genomic DNA Extraction - PureLink?
實驗概要The ?PureLink? Genomic DNA Purification Kit allows rapid and efficient ?purification of genomic DNA. The kit is designed to efficiently isolate ?g
Genomic DNA Extraction - Phenol | Chloroform
實驗概要This section provides a general protocol for genomic DNA extraction using phenol and chloroform.主要試劑1.?????? Glycogen (20 μg/μL)2.?????? 7.5 M NH4
Fungal DNA Isolation
Fungal DNA IsolationSaghai-Maroof MA, Soliman KM, Jorgensen RA, & Allard RW (1984) PNAS 81:8014-8018DNA successfully isolated from fungal species of C
DNA抽提
DNA抽提（主要內容如下）·???Working with DNA·???DNA Extraction from Bacteria and Other Organisms·???DNA Extraction from Cell and Tissue·???Mitochondria DNA Isola
DNA isolation extraction
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolati
Yeast Genomic DNA Prep
Grow 10ml YPD cultures o/n. Figure out cell density; inoculate 30 ml YPD and grow o/n so that cell density is approximately 2 x 108cells/ml the next