Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1a) is a tissue-specific coactivator that enhances the activity of many nuclear receptors and coordinates transcriptional programs important for energy metabolism and energy homeostasis. Inappropriate increases in PGC-1a activity have been linked to a number of pathological conditions including heart failure and diabetes. PGC-1a is highly expre......閱讀全文
AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of Helico
4. 總結及展望SIET在借鑒眾多科學家工作經驗的基礎上,經過多年的改進和完善,為科研人員提供了一個較為友好的軟硬件環境,在數據的生成,采集以及校準等方面,極大地方便了研究人員。特別是應用SIET強大的3維立體測量方式,研究人員可以獲得其他電生理技術無法測到的被測樣品某些點的特異活性 [10]。對于
51. Krupenina NA, Bulychev AA. Action potential-induced changes in photosynthetic pattern of a plant cell
Assay of superoxide dismutase activity by combining electrophoresis and densitometryAbstract. A modified technique was developed to assay superoxide d
AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEE
2019年上半年很快就結束了,iNature盤點了中國學者在Cell,Nature及Science發表的成果,我們發現總共有86篇(截至2019年6月24日),具體介紹如下: 4-6月發表的文章 【1】2019年6月21日,西北工業大學王文,中科院昆明動物研究所/BGI 張國捷及丹麥哥本哈根
TROUBLESHOOTINGProblem: The OP9 cells are more than 80%-90% confluent.Solution: It is important when creating working stocks of OP9 cel
7月31日歐盟委員會發布(EU)2018/1080條例,批準枯草芽孢桿菌 DSM 29784作為小型家禽飼料添加劑。 按照條例要求,該飼料添加劑在添加劑類別組屬于“畜牧添加劑”、在功能類別組屬于“腸道菌群穩定劑”。 該飼料添加劑在應用時,需保證其在水分含量12%的飼料成品中的最低含量為1×1
蛋白激酶PKS5通過阻斷質膜H+-ATP酶與14-3-3蛋白的相互作用抑制其活性關 鍵 詞:PKS5;質膜質子泵(PM H+-ATPase);非損傷微測技術(MIFE)參考文獻:Anja T.Fuglsang, et al. The Plant Cell, 2007, 19:1617-1634 全文
4. 注釋( 1 ) 每個實驗均使用新鮮的 3 mol/L 甲醇- HCl 和硅烷化試劑。( 2 ) 要仔細識別蛋白質印跡,因為 WGA 既能識別 N-糖苷的 GlcNAc,也能識別 O-GlcNAc。( 3 ) 用于在硝酸纖維素印跡膜上封閉結合位點的溶液應避免糖蛋白污染。所以我們建議在這一步驟中使
N-glycosylation modification of proteins has reported to improve the health of living organisms through antibacterial activity [68], antioxidant a
June 15, 2016 - Greifensee, SwitzerlandWith QbD-Quality by Design-weighing processes help ensure excellent consistency in final productsBy Tobias Woel
FluidFM 測定細胞粘附力的應用隨著時間推移,越來越多的學者開始使用FluidFM 技術進行測定細胞粘附力。以下就近五年的具有代表性的應用進行總結。Cohen 等使用FluidFM 技術對MCF7-MCF10A、MCF7-HS5 的細胞粘附力進行了測定,并與以往的文獻進行對比,發現其數據與Hos
【51/52】2019年4月4日,清華大學柴繼杰課題組、中科院遺傳發育所周儉民課題組和清華大學王宏偉課題聯合同期背靠背發表兩篇重量級Science文章,完成了植物NLR蛋白復合物的組裝、結構和功能分析,揭示了NLR作用的關鍵分子機制,是植物免疫研究的里程碑事件。兩篇文章分別是: "Li
湯森路透旗下的知識產權與科技事業部今天發布了2015年度引文桂冠獎獲獎名單,預測在今年或不久的將來可能獲得諾貝爾獎的科研精英。值得一提的是,華裔科學家王中林因近年來在納米材料可控生長、表征和應用等領域多項原創性研究成果而榜上有名。 自2002年以來,湯森路透已成功預測了37位諾貝爾獎得主。該獎
【50】2019年4月12日,中科院上海藥物所徐華強,王明偉,浙江大學張巖及匹茲堡大學醫學院Jean-Pierre Vilardaga共同通訊在Science發表題為“Structure and dynamics of the active human parathyroid hormone r
The hepatocyte growth factor receptor, also called c-Met, is activated by HGF and stimulates proliferation of hepatocytes and other cell types. Mutate
中國科學院大連化學物理研究所 許國旺研究員 來自中國科學院大連化學物理研究所的許國旺研究員做了題為“液相色譜-質譜用于代謝標志物的發現和鑒定”的報告。許老師提出,現在是一個組學的時代,代謝組學作為最主要的組學之一,目前在疾病研究、藥物研發及職務和微生物等領域均得到重視。 代謝組學是研
Telomerase is an enzyme which replicates the terminal sequences of eukaryotic chromosomes, namely the telomeres. Cells which have an unlimited replica
Cyclin-dependent kinase 5 (cdk5), a multi-functional kinase, and its neuron-specific activator p35 are required for neurite outgrowth and cortical lam
Significant progress has been made towards delineation of the intrinsic molecular processes that regulate B lymphocyte immune function. Recent observa
截至2019年12月31日,中國學者在Cell,Nature及Science在線發表了186篇文章,其中生命科學領域有109篇,材料學有30篇,物理學有20篇,化學有12篇,地球科學有15篇。iNature團隊對于這些文章做了系統的總結: 按雜志來劃分:Cell 發表了31篇,Nature 發
截至2019年12月13日,中國學者在Cell,Nature及Science在線發表了105篇文章(2019年的Cell已經全部更新完畢,而對于Nature及Science只剩下了一期,將分別會12月19日及20日進行更新),小編對于這些文章做了系統的總結: 按雜志來劃分:Cell 發表了30
實驗概要Interest in RNA-protein interactions is booming as we begin to appreciate the role of RNA, not just in well-established processes such as tran
The PML nuclear bodies are ring-shaped nuclear substructures associated with the regulation of transcription, transformation, cell growth, and apoptos
Hypertrophy associated with both hypertension and obstruction to ventricular outflow leads to pathologic cardiac growth and it is associated with incr
Over 1,000 papers and reviews have been written about the role of ceramide in the production of programmed cell death or apoptosis. Ceramide is a sphi
Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopyAbstractThe CRISPR-associated endonuclease Cas9
Glycolysis was one of the first metabolic pathways studied and is one of the best understood, in terms of the enzymes involved, their mechanisms of ac
生活中總能聽到平民英雄的傳說,其實在生物體內,也有著眾多無名英雄為了維護我們身體的健康無時無刻不在默默無聞的工作著。KLHL24就是其中之一。 導語 常態下,生為泛素連接酶的KLHL24,一面引領著獵物-KRT14一步不回頭地邁向深淵(蛋白酶體),一面又為維護人類的皮膚屏障不惜自行了